F. J. F. SHAW 47 
in two plants but they did not spread, and the plants remained living and healthy. 
This experiment took place during a period of relatively high temperature. 
All the above inoculations (Experiments [V—X) were made from a series of 
agar cultures which had origmated from a spore infection on agar in December, 
1917. The possibility of the fungus declining in the virulence of its capacity to 
infect the living jute stem owing to prolonged growth in artificial culture could 
not be lost sight of, and a further series of infections from a fresh isolation of the 
fungus was carried out. The fungus was re-isolated from spores during a visit to 
Dacca in August, 1918, and infected upon living jute plants in the field. The 
inoculations were carried out on a small plot which was sown with jute on 5th 
March, 1918, and the plants subsequently thinned out to a distance of 18”. This 
crop grew very well and attained a height of about 14 feet ; at the time of these 
experiments it was just over flowering period, i 
Experiment XI. Infected five plants on 9th September, 1918, as 
follows :— 
(1) A stem received a small tangential wound about 4 feet above ground 
level and was infected on the wound and the infection bound up with cloth. 
After 48 hours a brown stain was spreading from the seat of infection and 
pycnidia were produced and the plant rmged on I4th September, 1918. The 
plant wilted and died on 15th September, 1918. 
(2) A stem was infected on a small tangential wound about 4 feet from the 
ground level, but the infection was not bound up and was left exposed to the air. 
The plant was killed by 17th September, 1918. 
(3) Two stems were infected, each at the base of a small lateral shoot about 
4 feet above ground level, and the infections bound up with cloth. One plant 
died on 17th September, 1918, but the other never became completely ringed 
and survived. 
(4) One plant was infected at the base of a lateral shoot but the infection 
was not covered with cloth and remained exposed to the air. The infection 
spread with the usual symptoms but the plant was not completely ringed and 
did not die until 18th October. The plants killed in this experiment are shown 
rm PEW. fie:. I. > 
Experiment XII, Infected four plants on 11th September, 1918, as 
follows :—. 
(1) Two stems infected on tangential wounds and infections covered with 
cloth. Both these plants died—one on 19th September, 1918, and the other on 
25th September, 1918. 
(2) One stem infected on the stem surface and the infected section enclosed 
ina lamp chimney. This plant died on 25th September, 1918. 
