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MANORANJAN MITRA 69 
The medium gradually turns yellow in colour. Spore formation 
copious as in wheat broth agar. In measurement the conidia 
are 22°5—32°Ep x 10—ldz. 
18. Corn leaf juice agar (+8 Fuller’s scale). The growth is bad and 
almost entirely submerged. Very few spores are formed. 
19. Naegelis nutrient solution. Growth very poor. Very little 
aerial mycelium. 
20. Cohn’s nutrient solution. As in the above, here also growth is 
poor with very little aerial mycelium. 
From the study of the fungus in the above media it is found that some 
media are favourable for the formation of a large number of spores and some 
for the formation of chlamydospores ; while some are good for both. A few 
media are unfavourable either for growth or formation of spores and chlamy- 
dospores. In some media spores are nearly of the same size as those found on” 
the host under field conditions and in some they are much smaller. The 
perfe_t stage of the fungus does not occur on any of the above media, nor has it 
been found on the host. 
(d) The effect of reaction of media. This experiment was run in duplicate 
using glucose agar. Twenty tubes were inoculated, two of each reaction, 
ranging from—15 to+30 Fuller’s scale.* 
No spore formation. 
TaBLeE I. 
Tube No. Reaction | Gr pe REMARKS 
1 —15 Fullers Poor Growth poor but spore formation copious. 
scale 
2 —10 55 Fair In the beginning poor but after a few 
| , days fair. Spore formation normal. 
3 —5 | Fair | Growth was a little more than in —10. 
4 0 > | Good | Aerial growth in sufticiently large amount. 
3) +5 “ Very good | Aerial growth more than in 0. 
O | +10 ¥9 | Very good | Aerial growth more than in JU. 
7 | +15 re | Good | The growth in the beginning was poor. 
Se han eon Lease | Aerial growth little. 
y | +25 PA Poor No spore formation. 
lo | +-30 oe Very poo: | In the beginning there was no growth. 
* Media irom +15 to +30 remained ia the liquid condition on account of too mueh acidity- 
