MANORANJAN MITRA 67. 
a young culture. The plant was covered with a bell jar. There was also a 
control. In 24 hours 7 leaves showed sign of infection and the spots inoculated 
showed penetration of hyphe. After 48 hours three more were infected and two 
of the former spots had increased in size. On the fourth day infection took place on 
the remaining leaves and conidiophores began to appear four days later. The 
control showed no sign of disease. 
Experiment 5. (26-9-19.) Three shoots with ears were placed in small flasks 
containing water. Nine inoculations on leaves and nine on the spikelets were made. 
Three shoots with ears were kept as controls. All inoculations on leaves and ears 
were successful on the third day. The spots on the leaves increased very much in 
size and those on the ears also increased and infected the neighbouring 
spikelets. There was no sign of disease either on leaves or on the ears of the 
controls. 
Experiment 6. (26-9-19.) Ten inoculations on either side of the leaves and leaf-sheaths 
of two healthy young plants were made; spores only being used and the spots 
inoculated were covered with sterile cotton wool. The plants were placed outside 
in rain. A control was also kept. Six leaves took the infection in 24 hours and 
the remaining in 48 hours. The control remained healthy. 
Experiment 7. (26-9-19.) Twelve leaves of two healthy full-grown plants were 
inoculated and kept in the same way as Experiment No. 6. A control was also kept. 
Four inoculations were successful in 24 hours, two on the third day and the rest on 
the fifth day. The control was without any sign of the disease. 
Experiment 8. (11-10-19.) Twelve inoculations were made on four ears (3 on each) 
and the ears were covered with bell jars. A control was kept. After 48 hours a 
woolly growth appeared in the places inoculated and this went on increasing and 
infecting the neighbouring spikelets. Within ten days all the four ears were 
completely covered with aerial growth. The hyphe penetrated the glumes and pale, 
and in most cases even the ovary. The control was free. 
Experiment 9. (21+10-19.) Three ears were inoculated as above and a control was 
also kept. The result was as in the above experiment. 
Experiment 10. (25-10-19.) Three ears were inoculated in 9 places and the inoculated 
spikelets were covered with sterile moist cotton wool. After three days the cotton 
wool was removed and the spikelets were found to have turned brown in colour and 
on examination showed the presence of hyphe inside them. The control that was 
kept showed no evident sign of the disease. 
Experiment 11, (28-10-19.) Seventeen leaves and five ears of six healthy shoots were 
inoculated and the spots covered with sterile cotton wool. All except the control 
got infected in a week. 
Experiment 12. (28-10-19.) Five leaves and three ears of a grown up plant were 
inoculated and the inoculated spots covered with sterile moist cotton wool. All 
inoculations on leaves and ears were so successful as to produce the characteristics of 
the disease. The control kept was satisfactory. 
(c) Results of inoculations. Altogether 106 inoculations on leaves (both 
sides) and leaf-sheaths, together with 40 on ears, were made, and all were 
successful. The results of the above experiments prove beyond doubt the 
parasitic nature of Acrothecowm Penniseti on Pennisetum typhordeum. 
