APPENDIX, 
1. Glucose meat-extract agar. 
Peptone .. ts Bc a 1-0 grm. 
Sodium chloride ar Se aie 0:5 grm. 
A Meat extract He a ~ 0°5 grm. 
Glucose .. we ots * 4:0 grm. 
Water .. es Re -- 100°0 c.c. 
fe agar xe Ne ore 2.4 grm. 
Water .. os ne -. 100°0.c.c. 
2. Glycerine agar. 
It was prepared by adding 10 c.c. of pure glycerine to the above medium 
in place of glucose. 
3. Litmus lactose agar. 
It was prepared by adding 2 grm. of c.p. lactose and 4 c.c. of blue litmus 
solution to medium No. 1 in place of glucose. 
4. Starch agar. 
To 400 c.c. of hot water 5 grm. of pure starch were added and boiled for 
two hours. When a starch solution of 250 c.c. was obtained, it was 
added to 250 c.c. of the following nutrient solution. 
‘ Potassium phosphate $e +2 l grm. 
Magnesium sulphate a we 1 grm. 
A. Sodium chloride fe 6 7 1 grm. 
Ammonium sulphate. . oe o- 2 grm. 
\Water .. ae 3 oe LOOKcc. 
: f Calcium carbonate .. “2 5: 1 grm. 
= \ Water ben oe ie ve SgOOlcre: 
A and B were mixed. ae Be 
Starch solution 0 Ae -. 260 6.c; 
Nutrient solution .. 328 sro eZOUICrC; 
Agar e* - os es 5 grm. 
5. Dextrose agar. 
Dextrose oe ai 46 2 grm. 
Agar . te vie Ac 3 grm. 
Water .. ie ats -» | LOO ce: 
Nutrient solution as for starch agar oo) LOCICe: 
eres) 
