J. F. DASTUR 133 
enclosed within the seed coat, the germinating embryo is killed almost at once, 
but if it spreads when only a part of the cotyledons are within the seed coat, the 
seedling may escape total destruction from the disease and may grow into a 
healthy plant, especially if the weather is dry enough to check the further 
progress of the disease. 
Cultures of the parasite. 
Shear and Wood! have found sterilized cornmeal and corn-meal agar 
to be satisfactory media for growing the ascigerous stages of some fungi 
causing anthracnoses. Kriiger? succeeded in getting the perfect stage 
of Glaosporium musarum on potato stems completely or partly sterilized. 
Vermicularva Capsici has been cultivated many times on these media 
and on several others, but the perithecial stage has not as yet been obtainéd. 
In agar media the early growth of the fungus is slightly erial, white and 
floccose. As it grows it forms a thick leathery mat of interlacing hyaline 
hyphz on the surface of the medium. In four or five days after inoculation 
black raised bodies like pin-heads are visible. These are either the acervuli or 
sclerotia of the fungus. A band of black or brown stromatic tissue is formed 
where the agar slant touches the glass slides. As the culture grows old, stro- 
matic plates are laid down, closely in contact with the surface of the medium. 
The rial hyphe are uniform in thickness and do not bear conidia like 
those of Gleosporium piperatum. The matted hyphe on the surface of the 
medium and the hyphz in its substance vary a great deal in breadth. Some 
are extremely fine, while others are very broad. The cytoplasm of the broad 
hyphe, especially of those within the medium, is highly vacuolate. As the 
culture grows old, large hyaline vesicles appear as offshoots of the mycelium or 
short branches of the mycelium become very much enlarged at the tips. 
Daughter hyphe occasionally arise through the septa from within the 
parent hypha. All hyphe are hyaline except those that form acervuli, sclerotia 
and stromatic masses. 
The acervuli in culture media appear as round black bristly heads. They 
are identical with those found on the host itself. 
Pseudo-pycnidia. Acervuli, especially when old, are sometimes roofed 
over by a network of brown-coloured hyphz formed by the profuse branching 
of some of the setze and the marginal cells of the acervulus. 
1 Shear, C. L., and Wood, A. K. Studies of Fungous Parasites belonging to the 
Genus Glomerella. U. S. Dept. Agri. Bur. Pl. Ind. Bull. No. 252, p. 15, 1913. 
2 Kriiger, F. Beitraige zur Kenntnis einiger Gleosporien, I and II. Arb. Kais. Biol. 
Anst. f. Land-und Forstwirtschaft, IX, Pt. 2, p. 271, 1913. 
