M. MITRA 223 
A strain from the male inflorescence of maize was also isolated and inocula- 
tions were done on the leaves and spikelets of male inflorescences. All were 
infected and produced the disease as in fields. In this case all inflorescences 
inoculated were either enclosed in glass chimney or the plants were kept in 
moist chambers. Controls were kept on all occasions and remained healthy. 
Seedlings of maize were inoculated with a pure culture from leaves. The 
inoculation was done on the roots, grains and on the sheath and near the tip 
of the growing seedlings; within two days, in most cases, the sheath became 
yellowish brown and mycelium was found inside it. Later on, on examination, 
it was found that the mycelium had penetrated roots, pericarp and testa, and 
in some cases the aleurone layers of grains and also hypocotyl, etc. Most 
of the seedlings died on acvount of the disease but those which remained alive 
showed no growth of fungus in their tissue after some time. This shows that 
the fungus is capable of killmg and destroying germinating grains and young 
seedlings but the infection is localized and does not keep pace with the growth 
of the host as in smut. Details of inoculations are given in the following 
table :— 
Tape I. 
Summary of the inoculations on maize with H. turcicum Pass. 
No. of 
No. of | success-| 4. 
. : . No. of | Control 
Date Strain used | Place inoculated paar 7 A. caniteel linlacted REMARKS 
tions 
19-7-18 | Maize leaf Both sides of leaves 1 12 2 .. | Five leaves were 
wounded and 
seven unwounded. 
30-7-18 Do. Do. 15 15 1 .. | Leaves unwounded. 
5-8-18 Do. Do. | 9 9 i! at | Do. 
8-8-18 Do. Do. 4 4 1 ee Do. 
28-8-19 Do Do. 10 10 1 Do. 
5-10-19 | Maize male Do. 9 | 9 1 Do. 
inflorescence } 
17-10-19 Do. | Male inflorescence | ie 13 1 Do. 
19-10-19 Do. Do. pep” 85 1 Do. 
' 19-10-19 | Maize leaves Do. 15 | 15 1 Do. 
28-10-19 | Male inflo- | Do. 40 30 1 eae | Do. 
rescence | 
10-6-20 Leaf Leaves 6 5 1 Do 
7-8-20 Do. Do. 11 5 1 
10-12-20 Do. Do. 1l 10 1 
4-8-19 Do. Seedlings 22 22 10 | 
| 
Total | 192 174 2m | .. 91% 
