M. MITRA 227 
and the submerged mycelium is green to dark green. Spore formation is more rapid at a low 
temperature. 
(9) Potato juice agar (+6 Fuller’s scale). Growth not well developed. Hyphe cells 
show irregular swelling here and there. Spore formation is poor. Submerged growth greenish 
and aerial greyish in appearance. 
(10) Coon’s synthetic agar (+8 Fuller’s scale). The growth is quite good with woolly 
aerial mycelium of light greyish green colour. The medium also becomes greenish, spore 
formation is copious. Mycelium later on turns gemmate. 
(11) Cellulose agar (+9 Fuller’s scale). The growth is poor and almost submerged, of 
greyish colour. Spore formation fair. The colour of medium does not change. 
(12) Starch agar (+4 Fuller’s scale). Fair woolly growth, aerial hyphe arise in clusters 
and grow radially. The medium turns light green. Spore formation is good. 
(13) Corn-meal agar (+2 Fuller’s scale). The growth is best on this medium. Copious, 
woolly, greyish green aerial growth at 30°C. The medium turns greenish and then dark green 
on account of submerged growth. In four days at 30°C. the growth is very profuse and the 
tube is almost full, the mycelium spreading on the walls of the tube. Spore formation is very 
good especially at a low temperature at which growth is slow. Hyphee light olive green. 
(14) Oat-meal agar (+2 Fuller’s scale). Copious woolly aérial growth of greenish grey 
colour. The medium becomes dark green near the margin. The submerged growth is also 
greenish and then turns darker gradually. Spore formation fairly large in number and growth 
is more rapid at 30°C. than at 20—22°C. 
(15) Wheat-meal agar (+2 Fuller’s scale). Copious aerial growth, the mycelium spreading 
on the walls of the tube and lower portion of the tube quite full, Hyphz olive green, 
Abundant spore formation. 
(16) Rice-meal agar (+4 Fuller’s scale). Growth good, but little less than that on corn- 
meal agar. Copious spore formation. Lower portion of the tube quite full with mycelium. 
Medium turns light green. 
(17) Barley-meal agar (+3 Fuller’s scale). Very good woolly aerial growth. Mycelium 
looks dark greenish and hyphe light olive green. Abundant spore formation as on rice-meal 
agar. It is one of the best media for the growth and production of spores. 
(18) Jowar-meal agar (+2 Fuller’s scale). A good growth with abundant aerial mycelium. 
The medium gradually turns green and then dark green. Spore formation good especially 
at 20-22°C. Growth slow at low temperature. Spores generally are 60°8 to 140°6 by 19 to 
26-6 in diameter with 3 to 7 septa. Sometimes spores are very small and with few or no 
septum. Those abnormal spores are not more than 60s: in length and 10°4u¥ in breadth and 
are formed when kept at a little high temperature. 
(19 & 20) Sugarcane leaf juice agar (+4 Fuller’s scale) and rice leaf juice agar (+-3 Fuller’s 
scale). In both of these media growth is poor and submerged. Spore formation is scanty. 
(21) Maize leaf juice agar (+3 Fuller’s scale). Fair growth with abundance of spore 
formation. 
(22, 23 & 24) Sterilized maize stem, sterilized sugarcane megasse and sterilized paddy straw. 
There was a good production of spores on all, especially on sterilized maize stem and sterilized 
paddy straw. The cultures were kept for about 7 months in each case but there was no sign 
of the appearance of the perfect stage. 
(25) Sterilized wheat straw. A good deal of spore formation took place and greyish green 
mycelium was formed in the water at the bottom of the tube. No further growth of the fungus, 
however, took place though the tubes were kept for more than a year. 
(Note. Tubes in 22-25 were kept at two temperatures, wiz., 22°C., and at room temperature 
of about 27-30°C. The growth was the same at both temperatures.) 
The growth was best on corn-meal agar, barley-meal agar, rice-meal agar, Tha xter’s hard 
potato agar and jowar-meal agar respectively. 
