88 THE MIGROSCOPE. 
direction which the organic compounds have gone. In answer to the 
second proposition, Vine has found that the cell-wall is possessed of 
elasticity and porosity, which no doubt vary according to the charac- 
ter of the environment. With this knowledge the observer experi- 
ences little difficulty in realizing that it may be readily permeated by 
nutrient material presented in suitable form, or further by excre- 
mentitious matter, the retention of which would be injurious. 
MICROSCOPY.* 
Meruop or Investicatine Muscre.t—In his researches on the 
regeneration of cross-striped muscle, Leven first injected Flem- 
ming’s solution well into the muscle, then cut out a piece which, 
after further subdivision, was placed for some days in Flemming’s 
solution, and finally hardened in absolute alcohol. Sections were 
stained in four to eight hours, by a solution of saffron, made as fol- 
lows : 
Saffron, 1 part. 
Absolute alcohol, 100 parts. 
Distilled water, 200 parts. 
The sections were then washed in distilled water, and left in 
acidulated alcohol (O, 5 per cent. H. Cl) until they recovered their 
cle nuclei are pale with dark red nucleoli. Leucocytes take on the 
former yellow color. They were then treated with absolute alco- 
hol, oil of cloves, and finally mounted in damar. If successfully 
done, the karyokinetic figures appear deep dark red, while the mus- 
coloring matter more easily and keep it longer than the rest of the 
tissues, with the exception of the mitotic figures, a state of affairs 
very favorable for examination. The coloring altogether is so deli- 
cate and definite, and distinguishes so sharply between the various 
tissue elements, that any further staining is superfluous. 
Merxsop or Examinine Ants For InrestinaL Parasitic Inrusorta. 
—Mr. J. W. Simmons cuts off the abdomen of the insect, places it 
in a drop of distilled water, and teazes. Cochran’s crimson ink is 
recommended for staining the organisms, but any carmine ink would 
probably answer the purpose. Roseine is also useful. Osmie acid 
is employed for killing and fixing the infusoria. 
* Under this heading will be included descriptions of New Instruments, Micro- 
scopical Manipulations, Stains and Re-agents, Photomicrograpbhy, etc. 
+ Medical Chronicle, November, 1888.—From Trans. of 7th Congress for Inter. Med. 
Wiesbaden, April 9-12, 1888. 
