142 THE MICROSCOPE, 
now stain in a solution of iodine green until it becomes slightly 
green; quickly wash in water and mount in damar, taking care it 
does not remain too long in the alcohol. All glands, especially 
Peyer’s patches, are green, connective tissue red, submucous layer 
stands out bright red, mucous mucose and muscular coats yellow. I 
find in preparations steeped in OsO,, after hardening in er. acid 
mixture, various details are seen with equal clearness. 
A Moptrication or Batsam Mountine.—After staining and wash- 
ing the sections, I remove the water as usual by allowing the 
specimens to FLoaT in methylated spirit till it sinks by its own weight 
(NEVER PUSH SECTIONS UNDER); then place in weak alcohol; follow by 
absolute for some minutes; then place them on a slide and allow them 
just to become sodden; place a drop of clove oil beneath it by means 
of a sable hair brush (camel hair not stiff enough). The oil rises 
through the section and drives away the spirit. When all is given 
off, finish the clarification by touching the upper surface of the 
section with the oil. Mount in C. balsam or damar, ring, etc. This 
seems much more tedious than it really is, and, as I have never 
heard of it before, I venture to bring it before your readers. I tried 
it for sections arteritis obliterans and brain in syphilis, ete. 
Srarmninc Far Certs ry tHe Fascia or a Catr’s Necx.—After 
soaking for from one-half to three hours in a half per cent. solution 
of osmic acid the portion of the fascia is placed for fifteen minutes 
in a solution consisting of carmine, 4 drachm; borax, 2 drachms; 
water, 4 ounces. It is then washed quickly and mounted in gly- 
Garin, to every ounce of which two drops of formic acid have been 
added. 
Lornorus Crystaiinus.—Alcohol is used in mounting the 
above with the tentacles expanded. The spirit is blown as a spray 
upon the surface of the water containing the organisms; it mixes 
slowly, and the tentacles are thereby ot retracted. 
To Mount Permanent Stipes or Hamociopnin.—The crystalline 
mass may be spread out with needle on a warmed slide, then 
covered; or else treated (I) with absolute alcohol, (IL) spirits of 
turpentine and nited C. B.; or they may be mounted in pot. bichro- 
mate two per cent., and before applying cover-glass, allow to dry 
completely, then add Damar varnish and cover as usual. 
To Oxserve Sproutine or Spores Intro Bactitu1.—Drops of culti- 
vating fluid are placed on a series of cover-glasses; they are then 
inoculated with spores of the bacilli, inverted over glass cells, sealed 
with vaseline, and placed at a temperature of 36° C. Slides may be 
