THE MICROSCOPE. 191 
ANOPLOPHYRA AHOLOSOMATIS, a New Ciliate Infusorian Parasitic in the 
Alimentary Canal of 4 losoma chlorostictum, W. M., MSS. By Henry 
H. Anderson, B. A. Communicated to the Microscopical Society of 
Calcutta. Reprint. 
REPORT OF THE CENTRAL Park (NEW YORK) MENAGERIE, 1888. 
New Form or Posterior CoLporrapPHy. By J. H. Kellogg, M. D. Reprint. 
EXPERIMENTAL RESEARCHES Respecting the Relation of Dress to Pelvic. 
Diseases of Women. By J. H. Kellogg, M. D. Reprint. 
ON THE ETioLoGy OF DIPHTHERIA, an Experimental Study. By T. Mitchell 
Pruden, M, D., etc. Reprint. 
CORRESPONDENCE AND QUERIES. 
Tuscatoosa, ALA. 
Eprrors Microscope: 
Apropos of section-fixing, I would suggest the following slight 
variation of the method generally adopted in the use of the clove 
oil-collodion process, which I have found to combine the conveni- 
ence and readiness of appplication of a liquid fixative with the 
undoubted advantages offered by the dry-film methods, in that it 
allows the preliminary arrangement of the section, or a number of 
sections, on the slide, and the easy removal of folds and wrinkles, 
which latter, especially with large, thin sections, is often impossible 
if tissue must lie as it falls : 
Place the section (paraffin imbedded) on a perfectly clean slide. 
Arrange and smooth out folds with a camel’s hair brush dipped in 
alcohol. Hold an instant over alcoholic flame until paraffin partially 
melts and section adheres. Paint over section, and slide a thin film 
of the collodion mixture. Press down with the thumb a bit of 
tissue paper coated with same mixture, in the manner recommended 
by Dr. Reeves, to insure close contact. Planish with mounting 
forceps, remove paper, and place the slide on brass table, or water 
bath at the melting point of paraffin, until clove oil is evaporated, 
when section will be found firmly attached, and slide can be passed 
through benzol, alcohol, stains, etc., without danger of separation. 
Mayer’s albumen process can also be used as above, and is 
satisfactory. Frenzel’s gutta-percha and Threlfall’s caoutchouc 
methods are also reliable, but I think the collodion process, used in 
the manner described, is most available and most certain in its 
results, and I, for one, feel no need of a better plan. 
Very truly, K. D. Bonpurant, M. D. 
