THE MICROSCOPE. 239 
Case X.—“‘Haquisite vesicular Emphysema of the Lungs.” 
Anthracotic pigmentation of the lungs is strong; they are not 
especially indurated, the blood capacity is rather normal. The bron- 
chial and trachial lymphatic glands at the base are enlarged, indu- 
rated and slaty: some are adherent to the vessels and bronchia, close to 
to which they lie and the walls of which are streaked with black 
pigment. A larger black gland adheres firmly-to the principal 
branch of the artery pulmonalis. An incision discloses a small 
opening which leads into the interior of this gland. In the liver 
and spleen there is a deposit of black anthracotic pigment. 
To Prof. Dr. von Recklinghausen, who proposed and offered me 
the above problem for solution, and placed his pathological labora- 
tory at my disposal, so that I was able to make a thorough and 
scientific investigation of the same, I here offer my most sincere and 
heartfelt thanks. 
THE END. 
STRUCTURE OF MUSCLE. 
Vv. A. LATHAM, B. SC., F. R. M. 8. 
UNSTRIPED MUSCULAR FIBERS. 
SOLATION.—1. When the fibers are few and embedded in 
connective tissue, place a small piece of the tissue in 10 to 20 
per cent. nitric acid (H NO.) for a day; tease out in water. 
2. When the fibers form the greater part of the tissue, place it 
in 33 per cent. alcohol one or two days, or in 2 per cent. ammonium 
bichromate one day, and then water one day. The fibers may be 
stained with eosin, hematoxylin, borax-carmine or picro-carmine, 
preferably after they have been teased out. 
FIprILLAR STRUCTURE OF THE Fipers.—a.* Take the fresh stomach 
of a frog, prepare a section at right angles to the surface and in 
direction of its length, with aid of a freezing microtome or Valen- 
tin’s knife; mount it in 8 to 10 per cent. salt solution, and 
examine at once; or isolate the fibers by method (2) given above. 
b. Take the epiploon, or other thin, vascular membrane, of a 
rabbit; place it for a day in 2 per cent. ammonium bichromate; 
stain with picro-carmine, and examine the optical transverse section 
of the muscular fibers coating the small arteries: + 
ce. Stretch out the mesentery of a newt,{ and place it in 5 per 
* Englemann, Ouderz Physiol. Lab. Utrecht, Deel VI, Ast. 2, St. 4. 
+ Ranvier, Traité Technique et Histologie, p. 326. 
¢ Klein, Quart. J. Micros. Sc., N.S. XVIII, p. 315, 1878. 
