242 THE MICROSCOPE. 
when the fibers are warmed with 5 to 10 per cent. solution of 
ammonium chloride. It is best to mount a few fibers in a shallow 
glass cell, to cement the edges of the cover-slips, and observe the 
fibers from time to time. A small piece of muscle may also be 
placed in a watch-glass or a small beaker, and warmed with the 
reagent, and at intervals a few fibers taken and fixed with osmic 
acid, salicylic acid or alcohol. 
I find a good way is to place small pieces of the fresh muscle of 
frog or lizard in formic acid 25 per cent., until transparent; then into 
gold chloride, 1 per cent., 15 to 20 minutes ; formic acid, 25 per cent. 
for 24 hours in the dark; formic acid, 50 per cent., 24 hours in the 
dark; 20 per cent. formic acid glycerin, two to three weeks, until suffic- 
iently lightened in color. The connective tissue is now macerated 
sufficiently to let the muscle fibers easily separate. Mount in acidu- 
lated glycerin (1 per cent. formic acid). Muscles of hydrophilus 
may remain three hours in § per cent. chloride of gold solution. This 
method may be used to study the nerves of small arteries and veins. 
For fresh, smooth muscle (bladder of salamandra), a bladder is 
hardened in bichromate of potash, 1 per cent. or more. Remove 
the epithelium by penciling. Stain the tissues with hematoxylin, 
eosin or anilin, and examine in water (preferable to glycerin). 
To make the iris denser and more easy to split so as to show 
musclus dilatator papille, proceed as follows: Chromic acid, 0.01 
per cent.; chloride of gold, 0.1 per cent.; Palladium chloride. The 
iris may be stained by putting for some hours into strong acetic 
acid, and then with an acid mixture of carmine and glycerin. 
BretiocrapHy.—Arch. fur Mikros. Anat.; ibid., Zeit. Wiss. Zool. 
University oF Micuican, ANN ARBor. 
PROCEEDINGS OF SOC ETE 
THE SAN FRANCISCO MICROSCOPICAL SOCIETY. 
REGULAR meeting of this society was held June 26, 1889, 
with a large attendance of members. President Payzant 
occupied the chair. Frank L. James, M. D., and Prof. H. M. 
Whelpley, of St. Louis, were present as visitors; also, M. R. Rob- 
erts, of this city, and L. M, King, of Santa Rosa. 
The President announced, with regret, the death of F. L. How- 
ard, for many years a member of the society. 
Dr. James is well known to microscopists by his able contribu- 
tions to this branch of science. He gave an interesting account of 
a phenomenal class of crystals produced from salicine by the ex- 
