248 THE MIcROSCOPE. 
funnels of the vasa deferentia in both species are cup-shaped and 
furnished with abundant long cilia. The mature ova are of very 
large size, half the diameter of the body, and loaded with yolk 
spherules. In C. Arenarius they were found back as far as the 
13-15 segments; in the first and last of these segments was a single 
ovum; in the middle one, two. The ova were not freely floating in 
the body cavity, but were inclosed in distinct sacs, furnished with 
blood vessels. These sacs were perfectly independent of each other. 
There was no communication that could be traced between the 
ovisacs of adjacent segments. In C. Ater. there appeared to be a 
similar arrangement. The spermathece of the sexually mature 
worm offer a valuable differential character; they are more com- 
plicated in C. Arenarius. The spermathece of this species is 
divided into two parts by a median constriction. In that part which 
communicates with the exterior only are found the spermatophores. 
The distal pouch in Beddard’s specimen contained a liquid deeply 
stained by the coloring reagent. Its walls, moreover, were lined by 
epithelium of a different character to that found in the proximal part 
of the spermathecee. The spermathece extends beyond the tenth 
segment; in the sexually mature individual it reaches back as far as 
the thirteenth segment, where it traverses the boundary line between 
the twelfth and thirteenth segments; the next septum, between the 
twelfth and thirteenth, comes in close relation with the septum 
dividing the segments 11,12; at this point the septa almost fuse, 
and the spermathecze passes directly from segment eleven into 
segment thirteen. Where it traverses the two mesenteries, there is 
another constriction, but the epithelium does not change in character. 
MeCRO'S'C 0 Pv 
Scuuttz’ Mernop ror Staining anv Derectine Gonococcr.+—Pre- 
pare the cover-glasses in the ordinary manner and immerse them from 
five to ten minutes in a saturated solution of methyl blue in a five per 
cent. solution of carbolic acid. Wash in distilled water and immerse 
for a very few seconds in a very dilute acetic acid, one minim of the acid 
to the drachm of water. Washing in distilled water completes the 
process, though, if desired, a dilute solution of saffranin may be em- 
ployed as a complementary stain. Otherwise the gonococci will 
appear stained blue on a quite decolorized back-ground. 
*Under this heading will be included descriptions of New Instruments, Microscopical 
Manipulations, Stains and Re-agents, Photomicrography, etc. 
+St. Louis Medical and Surgical Journal, July, 1889, p. 45. From Muenchener Med. 
Wochenschrift. 
