THe Microscope. 343 
of the same size as those of the corresponding zone of motor 
areas, but are more nearly globular, possess a larger nucleus, 
and, in particular, receive their axillary process from below. Cells 
of this sort occupy the entire thivkness of the cortex to the 
depth of the giant cells, the only variation observed being an 
increase of size downward. Corresponding to the giant cells of 
motor areas are large pyramidal cells, each with a slender down- 
ward projection terminating in an axillary process, while the 
upper blunt extremity gives off numbers of fine fibrils. Thus 
the contrast is complete, the course of the chains of cells being 
completely reversed, and the author seems warranted in assum- 
ing that there is a similar variation in the direction of the stimuli 
traversing these chains. 
MICROSCOPY: 
NEW METHOD OF STAINING BactLLus TuBERcULI.—The Central- 
blatt fur Bakteriologie und Parasitenkunde gives the following, 
which it says is essentially the method of Von Kuhne, of Wies- 
baden. The staining agent is crystal violet (hexamethy] violet), 
and the contrast stain eosin. The violet is prepared as follows: 
No. de 
Crystal=wioletns 2c) ti concosdeaes oseee fOdasaeehe doce Ve bem 1 gm. 
PSELE DITO OE a) 5 seep hep cols SRM cs ae Pact, soa Seow ide Cane JOLGre. 
INo: 2 
FAITH OTA CATO ONAL cea actoee: os eceec eee ee tea ttenseneee 1 gm. 
Distill ccllswaten: 3 /ahs..ccssnetes oes eh ck er ae ..100 ¢. ¢. 
A quantity of solution No. 2 is poured in a dish, and enough 
of No. 1 added that a drop of the mixture placed on filter-paper 
gives a deep stain. This mixture is heated to boiling, and kept 
at that temperature during the operation of staining. For cover- 
glass preparations immerse no longer than one minute. Decol- 
orize in 10 per cent. nitric acid for four or five seconds. Wash 
in 95 per cent. alcohol, and counter-stain in 
Stain for half a minute in cold; dry, and mount in xylol 
balsam. P 
For sections: Stain one minute; decolorize in 25 per cent. 
nitric acid; wash in alcohol; counter-stain in eosin, and mount 
as before. This method, besides being rapid, is said to produce 
brilliant preparations.— National Druggist. 
