174 ^HE MICROSCOPE. 



out fear. Dr. Seiler says — "they are then placed in what is termed 

 a fixing solution, composed of — 



Hydrochloric acid (C. P .) i part, 

 Alcohol (95 p. c.) 4 parts, 



and allowed to remain therein until their color has changed from a 

 lilac to a rose color. The action of this fixing solution is to remove 

 the excess of color and to bleach all other parts, except the nuclei, 

 which retain the carmine staining in spite of the action of the acid, 

 so that they alone are brilliantly colored red." If it is desired to 

 have other parts retain the staining also, then a much weaker solu- 

 tion of the acid in alcohol should be employed. 



We have also used Dr. Seiler's Sulphindigotate of Soda. A few 

 drops are added to some alcohol and the sections placed therein. 

 Thus the sections are colored and dehydrated at the same time. 

 Double staining with aniline blue and carmine has given us beautiful 

 sections, especially of the ovary. We have sections that show the 

 graafian follicles, the discus proligerus, germinal vesicle, and germinal 

 spot, all together as clear as a diagram could picture them and 

 doubly stained also. 



A specimen was recently sent us from a neighboring state, with 

 the physician's statement, that the uterine discharges contained these 

 peculiar fibrils, "the fibres of a uterine tumor." Placed under the 

 microscope, the fibrils were seen to belong to the vegetable kingdom. 

 The physicitin is at a loss to account for the fact; we suggested that 

 perhaps his patient was using some decoction for a wash and would 

 not admit the fact to her physician. 



A new way oi mounting butterlly scales is to dissolve 

 one part of Anthony's " French diamond varnish " in two parts 

 of pure benzole. Apply a drop or two of the solution to a 

 slide, and in a few seconds, or as soon as the varnish has 

 set, press the wing of the butterfly gently upon the slide, 

 and then carefully lift it away. The scales will be found trans- 

 ferred to the slide in their beautiful natural arrangement on 

 the wing. Make a shalllow cell around the mounting and ap- 

 ply the cover-glass. Canada balsam must not be used, as it 

 disarranges the object. — yJf/i. Month. Mic. Journal. 



