ah EE al Royal Microscopical Society. 7 
It will be noticed from the same figure on Plate LV. that a new 
acquisition was obtained, in the form of a fresh fungoid growth; 
and this circumstance is important in showing the length of time 
that spores may le dormant in a solution before they become de- 
veloped. There can hardly be a doubt that the spores which gave 
rise to this new form of fungus were originally present in the malt 
extract from which the mould had been taken, and that they re- 
mained inactive until the process of decomposition in the organic 
matter had reached a poimt which perhaps was not only favourable 
to, but tended to stimulate their development. 
This, I believe, may be taken as an illustration of the difficulty 
that is experienced in making experiments with grain extracts, 
which are infested with animals and germs of various kinds to an 
extent that is something marvellous. 
A quantity of mould from lemon juice was next obtained, and 
added to a solution containing 14 per cent. of glucose, and placed 
in the same chamber in which the preceding experiment was con- 
ducted. On the sixth day the sample was found to contain 0°91 
per cent. of alcohol, and on the twenty-third day 8°44 per cent. of 
alcohol and -24 per cent. of acid. 
This mould was extremely persistent, and maintained its posi- 
tion on the surface of the liquid throughout the fermentation ; while 
the mould obtained from the malt became in a short time broken 
up and diffused throughout the sample. 
It is worth noting in the case of the lemon-juice mould, and I 
have remarked the same thing in natural wine fermentations, that 
the quantity of alcohol produced fully represents the percentage of 
glucose ; whereas in the case of the malt mould the quantity of 
alcohol produced is less than it ought to be according to the pro- 
portion of the glucose present. | 
In Fig. 7, Plate LY., is represented a specimen of the cells or 
spores taken from the residue at the bottom of the flask. 
A sample of pus was next introduced into a solution containing 
7 per cent. of glucose, and the sample was placed in a chamber kept 
at a temperature of upwards of 70° Fahr. 
On the seventh day the sample was found to contain small 
transparent cells or spores, and flocculent masses of mycelium were 
observed floating in the liquid. 
At the expiration of sixteen days the sample was found to contain 
0-65 per cent. of alcohol, and -06 per cent. of acid ; and it is some- 
what remarkable how free the sample was from microscopic animals. 
The mycelium and spores produced by the pus are represented 
in Fig. 8, Plate LY., and it will be observed that they are almost 
identical in character with the mycelium and spores represented in 
Figs. 3 and 4, on Plate LYV., and produced from the flour and malt 
albumen sugar solutions respectively. 
