318 PROGRESS OF MICROSCOPICAL SCIENCE.  [Mpnthly, Microscopical 
bacteridiz from the blood of an animal affected with charbon, using, 
in this instance, a bottle with a glass tube attached, as recommended 
by Hallier. The spores of this parasite were put into distilled water, 
and a few drops of this mixture were then forced into the jugular vein 
ofa colt. At the end of five days the animal remained, to all appear- 
ance, quite well. Five days after the first injection, two ounces of the 
same fluid, containing bacteridiz and micrococcus-cells, were thrown 
into the jugular vein of the same animal, by means of a large syringe, 
armed with a small needle-like canula. This was followed by symp- 
toms of fever, without loss of appetite, and the animal appeared soon 
to recover. On the tenth day after the inoculation, however, fever 
again set in, the appetite failed, the animal exhibited an unsteady 
gait, and died during the night. 
The following was the result of the autopsy : 
In the neck, around those spots where incisions had been made 
for the purpose of injections, the connective tissue was thickened and 
infiltrated with serum, exhibiting here and there ecchymosed patches. 
The jugular vein was filled with a dark-coloured blood of the con- 
sistence of tar, but otherwise not changed. Lower down on the neck 
there was well-marked serous infiltration in parts remote from the 
point of injection. On the left shoulder there was extensive extrava- 
sation of blood beneath the superficial integument, involving the 
connective tissue, and extending down beneath the muscles. In the 
abdominal and thoracic cavities and pericardium slight serous exuda- 
tion. Numerous ecchymosed patches in the peritoneum, intestines, 
pleure, heart, and lungs. Spleen enlarged, friable, and gorged with 
blood. Liver of a yellowish-brown colour, and the microscope showed 
beginning fatty degeneration in this organ. Kidneys studded with 
large, yellow, hardened and congested masses ; while fatty degenera- 
tion of the epithelial cells of the uriniferous tubules communicated a 
yellowish-grey colour to these entire organs. Lymphatic glands 
enlarged, softened, and several were of a brownish-red colour, caused 
by extravasation of blood. Brain and spinal cord infiltrated with 
serum, while the minute blood-vessels were distended with blood. 
Ventricles filled with a clear, colourless liquid. The blood was 
everywhere of a dark-brown colour, of the consistence of tar, and 
contained large numbers of parasitic growths characteristic of 
charbon. 
Here, then, charbon was produced in a colt by the injection of 
parasites taken from the blood of animals affected with that disease. 
In this instance the symptoms broke out on the ninth day after the 
injection, death occurring on the tenth day. 
Presence of Microscopic Bodies in Water.—In reference to this 
matter, Mr. C. Stodder has communicated a note to the ‘ Boston 
Journal of Chemistry’ for August. Referring to Mr. Metcalfe John- 
son’s views [in these pages], he says :—“ Having undertaken within 
a few months to ascertain the organic contents of the air in Boston, he 
commenced by drawing a large quantity of air through a small quan- 
tity of filtered water, which, when examined with the microscope, 
appeared microscopically pure. He found large quantities of organic 
matter, a very few organic forms, and a great many very minute bodies, 
