THE MICROSCOPE. 109 



trim the mass if necessary. Strong alcohol will soften celloi- 

 dine and dilute alcohol will allow it to harden. The specimen 

 may remain in the dilute alcohol until firm enough for cutting. 

 A sliding cut, using the whole edge of the knife, must be em- 

 ployed and the blade kept flooded with 50 per cent alcohol. 



Celloidine cannot be cut into very thin sections, but is useful 

 for tissues that would be injured by the heat employed in 

 paraffine imbedding. See Am. Mo. Mic. Journal; Vol. VII, 

 p. 229, for Minot's method in full. 



162. How shall I treat sections after staining in logivood, borax 

 carmine and the anilines ? — E. F. H. 



Logwood staining. After staining, simply wash in water con- 

 taining a trace of soda (alkaline,) dehydrate in alcohol, clear 

 with oil of cloves, drop on balsam and cover. 



Borax carmine. (Must not touch water). Wash in acid al- 

 cohol, (alcohol 5, hydrochloric acid 1,) then absolute alcohol, 

 clove oil, balsam. 



Safranin. Wash in alcohol, clear in turpentine, balsam, io- 

 dine green (methyl green,) wash in water, alcohol, xylol, xylol 

 balsam. 



Methyl blue. Wash in water 100 and acetic acid 1, then ab- 

 solute alcohol, cedar oil, balsam. 



Carbol-fuchsin. Wash in 25 per cent solution of sulphuric 

 acid in water, about half a minute, then in 60 per cent alcohol, 

 then absolute alcohol, cedar oil, balsam. 



Oil of origanum may be substituted for cedar oil, for clearing 

 aniline stained sections. 



163. Hoiv is the Ehrlich-Biondi triple-stain made? How is it 

 used for staining blood and tissues ? — Leslie Whalley. 



Methyl green (Iodine green,) * grain in 100 drops of water. 

 Acidfuchsin, \ " " 40 ( ' " " 



Aniline orange, (Safranin,) 2 " " 200 ll " " 

 Mix these solutions and filter. Spread the blood on a slide, 

 dry over a flame, stain in the above solution 10 to 15 minutes, 

 wash carefully in water, dry, and apply xylol balsam. 



For tissues, stain the section in the above solution for 12 

 hours, wash in water, dehydrate in absolute alcohol, clear with 

 xylol or oil origanum, drop on xylol balsam and cover. 



