60 The Microscope. 



Boil 1, 2 and 3 together, filter if necessary, add acetic acid so 

 that the liquid from purplish becomes distinctly red ; filter. 

 Stains nucleus red, when sections are put into alcohol, 50 to 70 

 per cent., made acid with a little hydrochloric acid. 



Borax carmine : carmine, 1 part ; borax, 4 parts ; water, 56 

 parts, the three forming one volume ; absolute alcohol 2 vols. 

 For decalcified bone, etc. 



BoRACic ACID CARMINE : Carmine, 0.5 grm.; boracic acid, 4 

 grms.; water 100 grms.; or carmine, 0,25 grms.; boracic acid 2 

 grms.; concentrated solution of alum, 100 grms. Boil either 

 mixture ; filter while warm. 



Ammonia carminate (dr}-) : 1, carmine, 1 grm.; 2, ammonia, 

 1 to 2 c. c; 3, water, 6 to 8 c. c; 4, chloral ; 5, strong alcohol. — 

 1, 2 and 8 heated together until the ammonia has passed oft ; 

 add 1 per cent, or 2 per cent, of chloral, then 4 to 6 vols, of 

 strong alcohol ; a precipitate forms which is washed with alco- 

 hol and allowed to dr3^ Keep in a well-corked bottle. 



Oxalic acid carmine: carmine, 1 part; ammonia, 1 part; 

 water, 1 part, the three forming 1 volume ; solution of oxalic 

 acid, 1-22, 8 vols. Dissolve carmine in ammonia and water; 

 add the acid and absolute alcohol, 12 vols. Stains rapidly. Clear 

 the sections in alcohol containing a little oxalic acid. 



Hydrochloric acid carmine : carmine, 4 grms.; alcohol 80 

 per cent., 100 c. c; hydrochloric acid, 30 drops. Heat on 

 water bath for ©ne-half hour ; filter while warm, and add a little 

 ammonia and filter again. Clear sections in alcohol containing a 

 few drops of hydrochloric acid. 



HEMATOXYLIN STAINS. 



Alum h.5:matoxylin : A, h?ematox3din cr3^stals, 1 grm., alco- 

 hol, 30 grms.; B, alum, 0.5 grm., water, 30 c. c. Drop A. into 

 B until the color is deep violet ; let it stand several days 

 in an uncorked bottle exposed to the light ; filter. Stains in 

 5 to 30 minutes ; wash with water ; overstaining reduced by 

 soaking in alum solution for from 4 to 12 hours. 



Glycerine hematoxylin : hematoxylin, 2 grms.; glacial 

 acetic acid, 10 c. c; gl3^cerine, 100 c. c; absolute alcohol, 100 c. 

 c; water, 100 c. c; alum in excess. Let the mixed solution 

 stand exposed to the light for some time ; filter ; keep in well- 

 corked bottle. Sections hardened in alcohol or potassium bi- 



