The Microscope. 51 



chromate stain in a few minutes ; can be used to stain before 

 sectioning. 



Glycerine h^ematoxylin : haematoxylin, 2 grms.; alum, 2 

 grms.; glycerine, 100 grms.; alcohol, 100 grms.; water, 100 grms. 

 Stains rapidly ; wash well with water. As a nucleus stain treat 

 the sections with alcohol slightly acid with hydrochloric acid ; 

 wash all acid out after its action. 



Aqueous hematoxylin (Mitchell's) : finely ground logwood, 

 2 ounces ; potash alum, 9 drams ; glycerine, 4 ounces ; water, a 

 sufficient quantity. Put the logwood in a large funnel with a 

 filter ; moisten the logwood and wash it with cold water until 

 the water comes through nearly colorless. Dissolve the alum in 

 8 ounces of water ; put a cork in the end of the funnel ; pour 

 the alum solution on the logwood in the funnel ; macerate 48 

 hours; remove the cork, drain off the water, and wash the log- 

 wood with enough water to make the solution up to 12 ounces ; 

 add the glycerine, shake, filter. Put a few pieces of camphor or 

 thymol in the solution. It will keep for a long time, and may 

 be used diluted. Stains rapidly; diluted stains more slowly 

 but perhaps more perfectly. 



Sections stained with hoematoxylin should be thoroughly freed 

 from all acid before mountinor. 



EDrroiCs,^ ^ 



DEPARTMENT 



^^f^: 



Ever}^ reader is more or less familiar with the theory of the 

 reticulation of the protoplasm within animal and vegetable 

 cells. Those that accept the teaching state that the proto- 

 plasmic contents of the cells can be shown to be formed of 

 interlacing threads, which together produce a network of great 

 delicacy. As good objectives become the property of micro- 

 scopists willing to do more than pla}'- in their leisure moments, 

 the theory of the reticulation of protoplasm becomes less and 

 less a theory and more and more a demonstrable fact, and 



