152 THE MICROSCOPE. [July, 



f. The introduction of the compound microscope in the exami- 

 nation of urine is by no means recent. The value of the instru- 

 ment in this work is so thoroughly recognized that it does not 

 admit of debate. The examination of urine as a part of a phar- 

 macist's work is fast gaining a similar position. 



g. In conclusion, there are many incidental uses for the com- 

 pound microscope in a drug-store, such as the examination of 

 the smaller insects which infest drug's, the study of moulds which 

 are found in pharmaceutical preparations, and other uses which 

 suggest themselves to pharmacists with an investigating turn of 

 mind. 



Double and Metallic Stains. 



Compiled by Professor A. B. AUBERT, 



ORONO, ME. 



Bismarck B7'ozvn a7id Methyl G7'een. — Bismarck brown, 

 a concentrated warm aqueous solution, or a weak alcoholic solu- 

 tion. Methyl green, 0.5 ; water, 100. vSections to be kept in 

 about 15 minutes; wash with water ; stain dark green, wash, 

 soaking in alcohol until grass green ; clear in oil of bergamot, 

 xylol ; mount in balsam. 



Borax^ CariniJie^ a7id Bidigo Ca7'77ii7ie. — i. Carmine, 2 

 grms. ; borax, 8 grms. ; water, 130 grms. 2. Indigo carmine, S 

 grms. ; borax, 8 grms. ; water, 130 grms. Mix i vol. of i and 

 2. Sections are taken from alcohol and stained in from 15 to 20 

 minutes ; transfer from 15 to 20 minutes to concentrated solution 

 of oxalic acid ; w^ash ; dehydrate ; mount in balsam. 



Eosiii a7id JSIethyl Gree7i. — I. Eosin, ipart; methyl green, 

 60 parts; alcohol, 30%; warm. 11. A. Eosin, i part; water, 

 50; alcohol, 50; B. Methyl green, i; water, 100. Sections 

 stain in i in 5 or 10 minutes ; quickly wash in successive alcohols, 

 mount in balsam or glycerine. II. Is for blood corpuscles ; dry 

 the blood (thin layer) on the slide ; treat with ^ 3 or 4 minutes, 

 wash stain oft' with water; treat with ^ 2 or 3 minutes, wash, 

 dry, mount in balsam (red corpuscles red, nuclei and leucocytes 

 bluish green) . 



Fuchsi7i a7id Methyli7i Blue. — A. Fuchsin solution in alcohol, 

 S to 10 drops; water, a watch-glass full. B. Concentrated 

 aqueous solution of methylin blue. Tissues to be hardened in 

 chromic acid, etc. ; stained in A for several to 24 hours ; washed 

 with alcohol, stained in B for 4 to ^J minutes (nuclei red, tissues 

 blue). 



HcE77iatoxyU7i a7td Eosi7i. — Solution of eosin in glycerine in 

 which some salt has been dissolved ; concentrated solution of 

 alum in glycerine, and glycerine haematoxylin. Stained speci- 

 mens washed in alcohol containing a little eosin ; clear in oil of 

 cloves ; mount in balsam. 



