APPENDIX. 



Agar Medium A. — 



Agar . . . . . . . . 15 grms. 



Ammonium nitrate . . . . . . 10 „ 



Calcium sulphate . . . . . . . . 5 „ 



Magnesium sulphate . . . . . . 1 ,, 



Lactic acid . . . • ■ . . 2 ,, 



Water .. .. .. 1,000 c.c. 



Agar Medium B. — 



Take 20 seeds of ground nut, grind them up in a mortar and boil with 150 c.c. 

 of water for two hours, allow to .stand for 24 hours, decant the supernatant liquid 

 and filter, add water up to .500 c.c. 



Agar . . . . . . ..75 gims. 



Aqueous e.xtract of ground nut _.. .. 500 c.c. 



.\gar Medium C. — 



Take 5 prunes, extract the stones and boil the prunes in 100 c.c. water loi 

 5 minutes, add water up to 500 c.c. 



Agar . . . . . . . . 7'5 grms. 



Extract of prunes .. .. .. •• 500 c.c. 



Agar Medium D. — 



Agar . . . . . . . . 15 grms. 



Ammonium nitrate 

 Calcium sulphate 

 Magnesium sulphate 

 Glucose 

 Water 



Agar Medium E. — 

 Extract of Lemco 

 Sodium chloride 

 Peptone 

 Glucose 

 Agar 

 Water 



10 „ 



5 „ 



1 „ 



20 „ 



1,000 c.c. 



4 grm 



5 „ 

 10 „ 

 20 „ 

 15 „ 



1000 c.c. 



