1 48 



\touritoi,onY amd pakasitism oi- uiiizoctoxia. 



Ayiir Mpdium F. — 



Take 50 ^riiis. of finely ;.'rouii(l French I)ean3, l)oil witli 10 e.c. of water for one 

 hour, and filter through a wirej^auze tea strainer. Add 10 ,i;rms. agar and water 

 up to 500 CO., boil and filter tliroujili linen cloth. 



Agar Medium (i. — 



Boil 30 <;rms. of ajjar with 1 litre of water tor 30 minutes, also !» <;rms. Salep 

 with 1 litre of water for 30 minutes. Mix the two together and add the following : — 



1 grm. (Jrape sugar. 



2 grms. Monopotassiuni piiosjiliate. 

 Tiiice .-Vmmoiiiiim nitrate. 



,, „ sulphate. 



„ Ferric sulphate. 

 ',, Magnesium sulphate. 

 2 grms. Tartaric acid. 

 Boil for half an hour and filter in the steriliser (see Mace. E.— Traite pratique 

 de Racteriologie, Paris, 188!)). 



Filler Pafer. — 



Ammonium nitrate 



Calcium sulpliate 



Magnesium sulphate 



Lactic acid 



Water 

 Take 50 c.c. of the above solution, add 10 grins, lilter \r.\\H 

 Kiister, K.— Kuitur der Mikronrganismen. 1907, [>. ltd). 



10 ''rms. 



1,000 r.f. 



anil sterilise (see 



