24 H. M. WOODCOCK. 



doubt, I think, that these Gregarines belong to Kunstler's 

 species. 



Triple association. — Not infrequently I came across 

 instances where the trophozoites possessed three nuclei. In 

 none o£ these cases was there any septum visible; they 

 evidently belonged to the category of very early associations. 

 Figs. 20, 29, and 28 show examples of such, the two first 

 being C. irregularis and the latter one C. minchinii. 

 The C. minchinii figured is in the gregariniform stage, as 

 usual, partly enclosed by epithelium. The middle nucleus is 

 not quite in the transverse plane of the other two ; neverthe- 

 less the three associates have, manifestly, all joined side by 

 side. In all cases the nuclei were of equal size and quite 

 similar in structure; there was nothing to suggest that two 

 were formed by one of the original ones dividing. 



I have also found a couple of instances of triple association 

 in Diplocystis Sclineideri (figs. 24 and 25). In fig. 25 

 two members of the triplet are slightly smaller than the third, 

 but in fig. 24 all three are about the same size.^ Similarly in 

 C. irregularis the three members are sometimes apparently 

 equal (fig. 20) and sometimes not (fig. 29), but the inequality 

 in the latter case is never pronounced. In C. minchinii, on 

 the other hand, the members of all the triplets I observed were 

 all quite equal. This equality is, indeed, only what one might 

 expect, since it may be said that the individuals cannot live 

 alone long enough to become appreciably unequal in size. 



(5) Minute Structure. 



(a) Nature of the peripheral region and composi- 

 tion of the septal plane. 



The minute structure of the peripheral part of the body 



^ Kuiistler (loc. cit., fig. 3) has drawn a triple association, and rather 

 insists upon the inequality of the members constituting it, regarding the two 

 smaller as resulting from the division of one large one. In my fig. 2i, how- 

 ever, scarcely any inequality is noticeable. 



