88 H. M. WOODCOCK. 



connected idea of the earlier processes in nuclear division in 

 C . irregularis; as to tliose in C. niincliinii, however^ I 

 am without any information. 



In fiof. 45 a is seen the earliest stao^e I obtained in this 

 nuclear preparation for sporulation^ and one which is most 

 important. Only one nucleus is shown in this section, the 

 other being further on ; a section of it is drawn separately 

 at h. Both nuclei are in exactly the same condition. Their 

 outline is slightly retracted and irregular, attributable to the 

 fact that the animal was fixed in a piece of the vascular net- 

 work, being still inside the lumen of the vessel.^ There is 

 no septum visible in this specimen, and it is evidentl}^ an 

 instance of precocious and complete union. 



The important point to notice is that the large, uniformly 

 vacuolated karyosome, as we saw it, for example, in fig. 36, is 

 no longer present as such. It is represented instead bj^ the 

 numerous small fragments of slightly varying size seen at/.; 

 they are fairly well stained, more deeply so at the periphery, 

 and stand out distinctly from the rest of the nucleus. The 

 karyosome has undoubtedly broken up or separated into these 

 little, more or less spherical, pieces, each of which jorobably 

 corresponds to one of the spherules of the original karyosome.^ 

 The nucleoplasm itself is distinctly chromatic, but not so 

 obviously reticular, being of a more granular nature, with 

 the granules of practically uniform size. There is not the 

 slightest appearance of any expulsion of karyosomatic material 

 into the surrounding cytoplasm, and the nuclear membrane is 

 perfectly entire. 



Further stages in the history of these karyosomatic frag- 

 ments are shown by the nuclei drawn in fig. 4G. These are 

 daughter-nuclei of the fourth generation, all belonging to the 



1 The relatively large size of the nucleus, as compared with the apparent 

 length of the body, is due to the fact that the sections passed obliquely 

 througli the Grogarine. 



' Compare Gregarina blattarum, whose karyosome forms a chain 

 ("cbapelet") of numerous tiny ones by successive buddings or divisions (see 

 Cuenot loo. cit., fig. 33). 



