LIFE-CYOLE OP " CYSTOBIA " TRREGOLARIS (mINOH.). 97 



Fig. 12. — Inpusliinw adult, to show especially the bending inwards of the 

 invaginated epithelium. (Tiie cytoplasm has shrunk away a little from the 

 limiting membrane.) Flemmiiig, picro-carmiue. X 250. 



Fig. 13. — Ditto, with a protrusion (.r) penetrating into a vascular strand. 

 (Nuclei of parasite are rather shrunk.) 90 % ale, para-carmine. X 150. 



Fig. 14. — Two sections of preceding, (a) Along line c— D; (b) along 

 A — B. lu the former the parasite is everywhere enveloped by the double layer 

 of epithelium. Thiouin + eosin. x 150. 



Fig. 15. — Section of parasite encysted in connective tissue around retractor 

 muscle. Corr. subl. + acetic, Klein, hsem. x 80. 



Fig. 16. — Section of another one, more imbedded. The inner layer of 

 epithelial nuclei distinctly seen at a. Flemming, iron-hsem. x 120. 



Fig. 17. — Section through part of an invaginated parasite (/;.), showing the 

 peculiar amoebocytes with peripherally situated nucleus («.) in the lumen of 

 the stalk. Iron-hsem. + orange, x 700. 



Figs. 18 and 19. — Sections through parasites in the wall of the respiratory 

 tree. Flemming, iron hgem. + orange, X 325. 



Fig. 20. — Evaginated C. irregularis; mounted whole. Triple associa- 

 tion. (The cytoplasm is rather retracted atone side.) Osmic + picro-carmine. 

 X 50. 



PLATE 3. 



Structure of Diplocystis schneideri. Triple association in Diplo- 

 cystis and " Cystobia "; precocious association.' 



Fio. 21. — Diplocystis schneideri, viewed whole. Flemming. x 50. 



Fig. 22. — A section through a couple. Corr. subl. -f acetic, iron-hsem. -f 

 orange. X 50. 



Fig. 23. — Another whole specimen. The endoplasm has shrunk away from 

 the outer layers (see text). Corr. subl. -f acetic, x 50. 



Fig. 24. — Ditto, triple association. The septal partitions are seen in two 

 places. Flemming. X 50, 



Fig. 25. — Another triplet, the members of which are not quite so intimately 

 joined. Corr. subl. -j- acetic, x 50, 



Fig. 26. — Nucleus of D. schneideri (from a section), m. Nuclear mem- 

 brane. Corr. subl. -f- acetic, Klein, hsem. x 200. 



Fig. 27. — Portion of the periphery of two couples of D. schneideri 

 showing the plane of junction (see text), sp. Space, (a) Klein, heem., (b) 

 para-carmine, after corr, subl. + acetic, x 600. 



Fig, 28, — C. minchiuii. Triple association, (The outer, free part of 

 the parasite is rather irregular,) Flemming, para-carmine, x 120. 

 VOL. 50, PART 1. NEW SERIES. 7 



