98 H. M. WOODCOCK. 



Fig. 29. — C. irregularis. Au evaginated triplet. This parasite lias a 

 somewhat altered, unhealthy look. Osmic, picro-carmine. X 150. 



Pig. 30.— Tiie smallest example of C. irregularis observed; already eva- 

 ginated. Tlie cytoplasm is scanty and without any granules. Corr. subl. 

 + acetic, iron-hsem. + orange. X 1000. 



Tigs. 31 and 32. — Ditto, of C. minchinii. Both in the wall of the 

 respiratory tree. n. Nuclei of tissue-cells. Flemmiug, iron-hsem. + orange. 

 X 1000. 



PLATE 4. 



Minute structure. Encystment and sporulation. 



Pig. 33.— Different nuclei of C. irregularis to show the different 

 degrees in the vacuolisation of the karyosome (seen whole). Osmic, picro- 

 carmine. X 400. 



Pig. 34.— Section through the nucleus of c. in the last figure (for descrip- 

 tion see text). Iron-hsem. -f eosin. X 700. 



Pig. 35. — Section through a typical nucleus (C. minchinii). Plemming 

 iron-hsem. + orange. X 500. 



Pig. 36. — Large nucleus and portion of surrounding cytoplasm of an 

 encysted C. minchinii. Klein, hsem. + orange. X 400. 



Fig. 37. — Portions of the periphery of two couples of C. irregularis, to 

 show the plane of junction (see text). Corr. subl. + acetic, iron hsem. -f 

 orange. X 600. 



Pig. 38. — (a) Periphery of C. minchinii to show cytoplasmic detail, 

 (b) Portion of cytoplasm of C. irregularis, (a) 90 % ale, (b) corr. subl. 

 -f acetic ; thionin + orange. X 600. 



Fig. 39.— Section through sporulating C. irregularis from the lumen of 

 the blood-vessel. Corr. subl. + acetic, iron-hsem. -f orange. X 80. 



Fig. 40. — Sporulating C. irregularis from same situation (seen whole). 

 More advanced stage. (The nuclei of this specimen were better stained than 

 usual, probably because it was not an " encysted " parasite.) Corr. subl. + 

 acetic, para-carmine. X 100. 



Pig. 41. — Section through encysted sporulating C. irregularis. iV. and n. 

 Large and small (sterile and germinal) nuclei. Corr. subl. -f acetic, iron- 

 iisbm. + eosin. X 250. 



Fig. 42. Ditto. This parasite was in a swollen, " spongy " evagination 

 (see text), of which to is the wall. .r. Amcebocytes and blood-cells. Flemming, 

 iron-hsem. + orange. X 120. 



Pig. 43. — Entire sporulating cyst of C. minchinii, attached to a fibrous 

 vascular strand. The cyst (seen in optical section) is full of ripe unstained 

 spores. Corr. subl. + acetic, para-carmine. X 85. 



