THE h;emoflagellates. 243 



growtli and nuclear multiplication. Following the process in an indifferent 

 form (fig. 30), it is seen that growth results principally in an increase in 

 length, and the ookinete becomes serpentine-like and then rolled and coiled 

 up upon itself (b and c), finally assuming the appearance of a tangled ball 

 or skein. Meanwhile nuclear division is proceeding. The nucleus here 

 remains for some time in the compound condition. Nuclear division takes 

 place in the same manner as in the megagametocyte of Trypanomorpha 

 (Halteridium) noctutu, and the kinetonucleus functions as a central 

 spindle. By successive divisions a great number of nuclei' are at length 

 produced, uniformly distributed throughout the coil (fig. 30 c). To each 

 daughter-nucleus a small zone of cytoplasm is apportioned, and each of 

 the "cell-territories" thus segregated becomes a little Trypanosome in 

 the way above described. By a similar process male and female ookinetes 



Pig. 31. — Liberation of young indifferent Trypanosomes from 

 the coiled ookinete in T. ziemanni. (After Schaudinn.) 



give rise at length to numbers of male and female individuals. When fully 

 organised, the young Trypanosomes, be they male, female, or indifferent, 

 liberate themselves and move away, leaving behind a large residual mass 

 of unused cytoplasm (fig. 31). 



When first set free, the young trypaniform parasites are very small, the 

 males being indeed, according to Schaudinn, hardly visible.- The indifferent 

 ones become greatly extended in length, and somewhat spirally twisted, 

 soon attaining the adult form. In all fundamental respects the organiza- 

 tion of the dift'erent types agrees completely with that of other Trypan o- 



' The number varies according to the type of ookinete. 



^ This is also true of the indifferent forms after repeated multiplication, 

 which can then only be made out when moving or agglomerated in clusters. 

 Schaudinn puts forward the interesting suggestion that there may possibly 

 be Protozoan parasites which, at certain periods of the life-cycle, can no 

 longer be optically resolved. Such may perhaps be the case in yellow fever. 



