THE HiEMOFLAGELLATES. 



245 



opposite ends are rounded and have lost the flagella. Multiplication also 

 goes on during this phase ; fig. 32 f shows four individuals, i. e. two couples 

 not yet separated. 



The behaviour of the indifferent forms, on entering the blood of the owl, 

 is very much the same as in the case of Trypanomorpha. After alter- 

 nation of resting, attached phases with multiplication periods has proceeded 

 for some time, sexual individuals are developed in increasing numbers from 

 the young indifferent forms. Sexual forms (gametocytes), whether male 



Pig. 33. — Active and resting phases of the gametocytes of T. 

 ziemanni, a — c, megagametocyte (female Trypanosome) ; d, e, 

 microgametocyte (male Trypanosome). (After Schaudinn.) [In 

 D, the myonemes are rather too accentuated.] 



or female, are easily distinguishable by the remarkable size to which 

 they grow, becoming, as they do, very much larger than the leucocytes. 

 An adult fully-grown male individual, or microgametocyte, in the try- 

 paniform phase is seen in fig. 33 d-. Both trophonucleus and kineto- 

 nucleus are very prominent, and the undulating membrane and flagellum 

 are well-developed ; the latter extends some distance beyond the posterior 

 end of the body. The sixteen myonemes are arranged in four double rows 

 or pairs on each side. A female Trypanosome (megagametocyte) in the 

 same phase (fig. 33 a) is even larger than a male form, but its nuclei are 

 relatively smaller, and there is no free prolongation of the flagellum. The 

 myonemes of each side are not arranged in pairs. 



