250 H. M. WOODCOCK. 



In addition the author describes various nuclear changes and divisions 

 undergone by the parasites (both forms) while in the blood of the 

 Vertebrate host. These include a regulatory process, characterised as 

 autosynthesis of the karyosome (karyocentrosome) and partheno- 

 genesis. • 



Equally interesting is the evidence already to tand, wliicli 

 tends to prove that the role played by an Insect in connection 

 with the Trypanosomes of warm-blooded Vertebrates is per- 

 formed by a leech in the case of those of cold-blooded 

 Vertebrates. 



To Leger (66 and 67) we owe certain instructive observations 

 relating to Trypanoplasma varium and Trypanosoma 

 barbatnlfe from the loach.^ This investigator distinguishes 

 indifferent and female forms of Trypanoplasma varium 

 in the blood of the fish. When a leech (Hemiclepsis 

 margin ata) sucks blood containing the parasites, which 

 thereupon pass into its stomach, the indifferent forms dege- 

 nerate and perish, while the female ones become massive and 

 show nuclear changes (division of both tropho- and kineto- 

 nucleus), preparatory, Leger thinks, to a sexual process. At 

 any rate, after some days the intestine of the leech contains 

 little, narrow Trypanoplasma t a, of which certain, very 

 filiform, ones represent, perhaps, male forms.^ Other stages 

 were also noticed whose interpretation remains at present 

 doubtful. In the case of Trypanosoma barbatul^e the 

 resemblance between the development of the parasites in 

 another leech (a Piscicola) and that of Schaudinn's Avian 

 forms in the gnat is still more pronounced. Eighteen hours 



1 It may be pointed out that certain of the appearances depicted as 

 representing different stages in these processes do not suggest, quite so 

 readily as might be desired, the interpretation given of them. In one or 

 two cases, at any rate, the figures recall those of Bosc (5), who describes 

 some wonderful phases in the development of a Trypanosome from the 

 rabbit which the writer, however, does not for a moment consider are really 

 normal. 



- Only a short preliminar}' note without figures is as yet available. 



' Brumpt (10) has also noticed small, very motile Trypanosomes in this 

 leech. 



