272 H. M. WOODCOCK. 



Trypauomorpha noctua3 and Trypanosoma ziemanni. 

 Hence, it may be taken for granted that agglomeration 

 normally occurs by the sensitive pole. It cannot but be 

 regarded as highly significant, therefore, that, in all species 

 of Trypanosoma for which this property has been ascer- 

 tained, the union is by the non -flagellate end; in the 

 writer's opinion, this evidence to a large extent compensates 

 for the paucity of observations relative to attachment. Up 

 till now agglomeration has not been observed in Amphibian 

 or Piscine forms. 



(3) Conjugation. — It is difficult to imagine a micro- 

 gamete, which exhibits definite cell-polarity, coming into 

 contact with the female element, prior to union or penetra- 

 tion, by any other than the sensitive, anterior pole. Hence, 

 in the case of the Hasmoflagellates, the end by which a 

 microgamete (a male Trypanosome or its homologue, it is to 

 be remembered) conjugates with a megagamete is of great 

 interest. Unfortunately, very few instances of fertilisation 

 are, so far, to hand. From Schaudinn's account, the process 

 in Trypauomorpha noctuse fully bears out the evidence 

 afforded by other considerations, and the same is probably 

 true of Trypanosoma ziemanni^; a noteworthy point in 

 the former case is that the flagellum of the microgamete, 

 being at the anterior, penetrative end, is not developed. 

 The only other case known is that of T. lewisi (Prowazek 

 [I.e.]), to which reference has been made. Granting, 

 as is very probable, tbat this author's description and 

 figures (see above, p. 249j represent the manner in Avhich 

 true conjugation occurs, the question of the correct orienta- 

 tion of tlie body in this form appears conclusively settled in 

 favour of the view here taken. 



(4) Movements of Investigation. — In this category 

 are to be placed the slow creeping or crawling movements 

 which have been observed in certain Trypanosomes (see 

 Section VII (a) ). Both the apparently tentative or inves- 

 tigatory character of the movement, and the fact that it is 



' bee footnote, p. 217. 



