HABITS OF ANOPHELES RONDONI IN ARGENTINE REPUBLIC. 454 



Ex-perijnent II. ]March 7, 1927. Fed batch of mixed mosquitoes on case of 

 tertian malaria. This man had taken 1 gram of quinine sulphate 24 hours previously, 

 but the blood still showed a few gametocytes. March 8. Allowed the mosquitoes 

 to feed on a second case of tertian malaria; this patient had taken I5 grams of 

 quinine sulphate 48 hours previously, but still harbored gametocytes. Some of the 

 batch fed on both occasions and all fed at least once. March 16. Dissected all of 

 the mosquitoes then living: 7 A. rondoni and 6 A. pseudopuncHpennis — all negative. 



Experiment III. April 23, 1927. Fed batch of mixed mosquitoes on case of 

 aestivo-autumnal malaria. This patient had taken 1§ grams of quinine sulphate 

 24 hours previously; the blood was rich in gametocytes. There fed 8 A. psendo- 

 punctipenyiis and 5 (possibly 6) A. rondoni. May 4. Dissected all of the mosquitoes. 

 A. rondoni all negative. Three A. pseudopundipennis found with stomach cysts as 

 folio ws: one with nine cysts, all large and well along in sporozoite development; 

 ene with eighteen cysts of variable sizes, but even the smallest showing radiate 

 structures; one with eight cysts, all retarded in development — small, with no visible 

 internal structures except coarse granules of dark pigment. 



The results of the feeding experiments are interesting not only to 

 compare the infectivity of A. 'pseudopundipennis and A. rondoni, but 

 further to emphasize the fact that P. falciparum gametocyte carriers 

 are still dangerous transmitters after taking quinine, while the same 

 appears not to be true of P. vivaz carriers. 



Experiments in rearing adults from eggs. 



These ma}^ be briefly summarized by saying that five batches of 

 eggs laid by known A. rondoni mothers captured in March were carried 

 through to the adult stage, the emergences being respectively 52, 26, 

 24, 8 and 1, a total of 111. These were all perfectly typical A. rondoni. 

 In addition, two batches of A. tarsimaculatus from females secured in 

 December and two from females caught in March were bred out, the 

 emergences being, 1, 6, 1, 6, a total of 14 from the four batches. The 

 young were all typical tarsimaculatus. The mothers in both series 

 {rondoni and tarsimaculatus) were caught in Cañitas, Ledesma. 



Although the emergences of A. tarsimaculatus were too small to be 

 conclusive, the indications are that both tarsimaculatus and rondoni 

 breed true, the male parents in every case being unknown. Of course, 

 there is the remote possibility that further breeding experiments would 

 indícate crossing. The experiments done so far confirm specificity as 

 determined by size and morphological characteristics. 



No adult A. rondoni emerged under 26 days after the eggs were 

 laid; nearly all were out by the end of five weeks, but a few larvae in 

 one batch lingered 44 days and died without pupation. This was in 

 March-April, 1927 (late summer and earh^ autumn). Sex was noted 

 in 107 of the 111 bred out: 58 females and 49 males. Among the orig- 



