151 



same way as iu the preceding test aud tbe reactions obtained were some- 

 what similar. 



Reagent 1. A precipitate was formed, but it was not so marlied as in 

 the ergot of rye. 



Reagent 2. Reaction much the same as that in rye ergot. 



Reagent 3. Reaction not quite so marlied as in tbe rye ergot. 



Reagent 4. No reaction was obtained. 



A brief summary of the work shows that a substance was obtained iu 

 all the extracts made which gave positive reactions with the first two 

 reagents used and negative ones with the last two. 



Commercial extracts of rye ergot and of corn smut gave similar reac- 

 tions to those obtained from the corn smut extract prepared in the lab- 

 oratory, in the case of reagents one and two, and in addition gave marked 

 results with reagent three. 



PHYSIOLOGICAL EFFECT OF AN ALCOHOLIC EXTRACT OF 

 CORN SMUT UPON HORSES. 



Tbe study of the physiological effect of an alcoholic extract upon 

 horses was carried on in conjunction with that of the alkaloidal tests in 

 the laboratory, the alcoholic extract used being prepared by the writer 

 in the same manner as that described in the preceding pages. The experi- 

 mental work upon the horses Avas performed by Dr. R. A. Craig, of tlie 

 Veterinary Department of Purdue Universitj'. 



The appended notes upon the amounts and effects of the doses admin- 

 istered were taken by him and have been kindly placed at my disposal. 



Horse No. l.—A gelding, poor in flesh, but healthy, was given 15 cc. of 

 the extract subcutaneously. The dose seemed to have no effect. The 

 next day 30 cc. were given in the same way. In twenty-five minutes he 

 stopped eating. The pulse and breathing were quickened and the peri- 

 staltic movements of the intestines were increased. Forty-five minutes 

 after the drug was given faeces were passed. No further effects were 

 noted. 



Horse No. 2.— A gelding in good condition was given 25 cc. subcutane- 

 ously. In twenty minutes he became restless, stopped eating, and the 

 pulse and breathing were quickened. A moist evacuation of faeces oc- 

 curred in twenty-five minutes. An hour after giving the injection its 

 effects had passed off. Two days afterwards 45 cc. were given. The horse 



