93 



member of the party. Mr. J. E. Guthrie, devoted nearlj- all his time to 

 insects, of which he secured a large collection. Another member of the 

 party gave special attention to leeches, and a third collected and studied 

 the mammals. The Avriter devoted nearly all his time to a study of the 

 fishes. Collections vs'ere made of the species found in the lake and the 

 inflowing streams, and many of the species were dissected and photo- 

 graphed. Attention was also given to a study of the habits of these 

 fishes. From the main camp expeditions were made to all parts of the 

 lake. 



We were on the lake a1)out two months and a half, and found the 

 camping method a very satisfactory and pleasant one. The limit of this 

 paper will not permit me to state any of the results of our Avork, but they 

 will appear later in the forthcoming reports of the survey, some of which 

 are now under way. 



Culture of Amoeba. 



A. J. BiGNEY. 



Several years ago I presented a paper l>efore this Academy on a new 

 method of obtaining amoeba. This method was as follows: Take some 

 of the green scum from the surface of some ix>nds and place it aside for 

 a few weeks, during which time great numbers of amoeba will have 

 developed. This scum must be composed mostly of euglena in the resting 

 stage. I have never known this method to fail. 



During the past season I have had unusual success Avith this method 

 of securing them. About the first of September I obtained some of these 

 euglenae and placed them in a wide-mouthed two-ounce bottle and left 

 them on my desk for about two months, at which time I needed them for 

 class use. When I first took this material from the pond there were a 

 few amoelta in the midst of the euglena. When I needed a supply I found 

 them by. the hundreds on almost every slide. Frequently there would be 

 so many that they would literally fill the field of the microscope. They 

 were large specimens. It was the largest supply that I have seen reported 

 in this country. It may be that others have had equal success with some 

 other method. We used them for some time and then nearly all the re- 

 maining ones went into the resting stage. 



