274 



which was four years and six mouths of age, the cultures from wort- 

 gelatine, rice and the original material having failed. 



For a fifth series of cultures gelatine was added to beef broth, and the 

 results of these cultures were the six from wort grew, the fungus being 

 in a vigorous state, also one from wort-gelatine, which was three years 

 of age, the growth not being vigorous; cultures from bran, rice, original 

 material and the remaining four of wort-gelatine failed. 



The series of moist chambers in which a drop of wort was used was 

 then made, and the following were the results obtained: Germination had 

 taken place in cultures obtained from rice three years and nine months 

 of age, one from wort-gelatine thi-ee years of age and the six from wort. 

 Those failed which had been obtained from cultures on bran, dextrose, 

 original material and the remaining four on wort-gelatine. 



A series of cultures was also made using Pasteur solution and alcohol 

 to normal solution, but no results at all were obtained, germination having 

 failed in every culture. 



New cultures were made in wort from the original cultures which had 

 germinated in just one or two testing media and were as follows: One 

 from wort-gelatine which was three years of age and had gei-minated in 

 beef broth and gelatine; one from rice three years and eight months of 

 age, which had germinated in the moist chamber, wort having been used; 

 one from bran four years and six months old, which had germinated in 

 bouillon; one fi-om wort-gelatine three years old. It had germinated in the 

 moist chamber. These four cultures failed to give any sign of germina- 

 tion. From this result it is suggested that the cutting off of the air supply 

 had permitted the conidia to germinate in wort in the moist chamber 

 where the test tube cultures in wort failed to promote germination. 



A microscopical examination was made of the conidia from cultures 

 of various media and dates, the following being the conidia examined: 

 Those from original cultures in wort, wort-gelatine, dextrose, from cul- 

 tures of Series I, in which wort was the medium; Series III, Pasteur solu- 

 tion the medium; Series IV, bouillon the medium. In these examinations 

 the conidia showed no apparent difference. 



The tabular form of these experiments and the results obtained from 

 them suggests the following conchisions: 



First.— The germinative power of the conidia of Aspergillus oryzae is 

 dependent upon the medium upon which the inoculating material has been 

 groAvn. 



