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bad matiiretl. but a warm rain bad uo effect. Fresb flowers placed in a 

 refrigerator were not affected by tbe reduction of tbe temperature, wbile 

 tbose exposed to cold rains seemed to bave tbeir irritability diminisbed. 

 Tbe application of pollen from tbe same or anotber flower had no effect 

 wben care was exercised not to rougiily touch the stigma. Pollen was 

 applied one evening to the tip of the lower lobe, which is the larger and 

 longer of the two lobes, and it did not binder their opening next morning. 

 After closing up from tbe use of an irritant alone they opened again in 

 about two hours. But if the irritation bad been accompanied with the 

 application of pollen from the same or another vine they rarely opened 

 again, and never if tlie ovary was fertilized. 



More than fifty experiments to determine the effects of pollenization 

 with pollen from the same flower as the stigma treated, or from another 

 flower growing on the same stock, gave negative results. In some cases 

 the ovary seemed to swell and remained attached to tbe vine longer than 

 those not pollenated, but they all turned black or dropped off within 

 fifteen days. 



All the stigmas treated, to determine their irritability, and the effects 

 of pollen applied to them coming from a distance, grew on vines in the 

 back yard of No. 823 Washington Street. Columbus, Indiana. Six strong 

 stocks, coming from the same root, cover the fence and an old apple tree. 

 In the autumn of 1901 they produced many matured capsules. August 

 the 10th and 20th, 1902, eleven stigmas were pollenated from flowers col- 

 lected two and one half squares distant. Six of these began to develop in 

 fine style, but came to naught. September 9tb and 10th, six stigmas vrere 

 treated with pollen from a vine found growing outside tbe city limits, one- 

 fourth mile west on the Nashville road. As a result, the ovary, in one 

 instance grew to be one inch long and then withered. The others were 

 failures and tbeir ovaries did not appear to bave grown a little bit. The 

 season closed with nothing to show for my work and the distance theory 

 unverified. The vine in my yard began blooming again July 1st, 1903, and 

 the first experiment of that year was made to see how much influence the 

 soil in which the vine grew had to do in determining the final results of 

 cross-fertilization. July 5th, I collected flowers from a vine growing in 

 the rich bottom land of Clifty Creek, two miles south of the cit.y, and 

 twenty stigmas growing in my yard were pollenated. The flowers treated, 

 were in all stages of blooming, from those just opening to others that 

 were fading, Imt none where the lobes of tbe stigma did not promptly 



