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close when irritated. These exiieriniputs resulted in twelve full grown 

 capsules. July 31st and August 3d eighteen stigmas were pollenated from 

 flowers found growing on clay soil, one mile south of the city, which, 

 resulted in three mature capsules. August the 14tli and 18th, nineteen 

 stigmas were treated with pollen from a vine growing in clay soil one- 

 half square north of my vine, and three mature pods were the result. 

 Ten stigmas were pollenated August lOth from a vine growing in clay soil, 

 at the root of a large elm tree, about one square northwest of the home 

 vine, and eight matui'e capsules were the result. 



Summarized, the lesults show that sixty per cent, of the polleu.ations 

 made with pollen from a vine growing in rich loam were successful; 

 fifteen and sixteen per cent, were successful when the pollen came from 

 clay soil, and the vines grew in the open, under conditions nearly the 

 same as that of my back yard, and eighty per cent, as the result whea 

 the pollen came from a vine whose roots were planted in clay soil and 

 eutertwined with those of a big elm. From this it seems that the soil in 

 which the vine grows, has some influence on the fertilizing power of its 

 pollen. The pollen used in the 1902 experiments, which resulted in fnil- 

 ures, came from vines growing in the open and rooted in clay soil. Th^; 

 idea that pollen coming from the big elm tree vine is in some way pecu- 

 liarly efficacious in prcdiicing seed is confirmed by the fact that a vine 

 within one hundred yards of it, and favorably located to encourage hum- 

 ming-birds to visit betAveen the two, has lorne an abundant crop of cap- 

 sules for the past two years. 



The only insects noticed on the trumpet-flowers were rol)l)ers. whose 

 visits were without compensating advantages. Black ants and little 

 sweat bees came early and stayed late; the ants to get nectar, and the 

 bees to collect pollen. Sometimes they found an entrance between the 

 lobes of the corolla limb before the flov.-er was open. The bees made short 

 work of collecting all the pollen in sight — half of it going within fifteen 

 minutes. When the pullen Avas knocked down into the tube they did not 

 seem to be in any way put out, but Avent on collecting until all was gone. 

 As many as six bees Avere seen together in a corolla, very busy, croAvding 

 and fighting for place. Had they found any pollen on a stigma they 

 would have taken it. During a drouth conical holes were found in the 

 calyx, of many floAvers, that reached doAA'u to the ovary, and as mud- 

 dauber wasps, t^phcgidc. Avere seen about the holes they Avere charged Avith 

 making them. After r;iin came they disappeared, and may have done the 



