BUREAU OF ANIMAL INDUSTRY. 



647 



vaccination when tliey were fed, and the tiino intervening was suffi 

 cient for tlie development of the disease from the injected virus. 



Pigs Nos. 151, 1(50, 170, and 178 were inoculated as in the preced- 

 ing experiments on February 8 and '^:i with ^''*' of a beef infusion 

 peptone culture derived from a guinea-pig and the seventh culture 

 from the spleen of a pig in the same medium. The dose was diluted 

 in salt solution so as to make l'^*^ of liquid. In No. 151 the second 

 inoculation produced a tumor about 1 inch long and i inch thick. 

 The iirst was scarcely noticeable. In No. 109 the first inoculation 

 resulted in a bean-like nodule;^ the second produced several of the 

 same size. In No. 170 neither inoculations showed more than a very 

 slight swelling. In No. 178 both inoculations produced rather exten- 

 sive swellings. 



On being- fed Avith the viscera of pigs known to have died of the 

 disease all took the disease and died; two on March 13 and the re- 

 maining two on March 10, one in* thirteen, one in eighteen, and two in 

 twenty-two days after feeding. A table summarizing these facts and 

 brief j906'^ mortem notes are appended: 



Autopsy notes. — No. 151. — Purplish spots on skin of abdomen and palei* ones in 

 subcutaneous tissue. Inoculation tumor cuts like cheese; yellowish white. Extrav- 

 asations vmder endocardium and epicardium; left lung mottled from congested 

 areas; cortex of lymphatic glands congested; those of meso-colon and lesser curra- 

 ture of stomach dark purple throughout; kidneys pale; hemorrhage into pelvis of 

 left kidney; extravasations into mucosa of stomach; moderate number of iilcers in 

 caecum and colon; large quantity of blood in the lower six or eight feet of ileum and 

 in the large intestine; clotted in the former tube, where the mucous membrane is 

 deeply congested. 



No. 169. — Small tumor on the left side, the place of the second inoculation; spleen 

 enlarged and congested, with large hemoiThagic infarcts; considerable effusion in 

 the large serous cavities. Besides the general congestion of lungs, there are scat- 

 tered throughout its parenchyma hemorrhagic foci. Hemorrhagic inflammation of 

 kidneys manifested by bright red glomei-uU throughout its cortex; lymphatics in 



feneral deeply congested; numerous petechias in stomach, small and large intestine, 

 n caecum and colon large, deep ulcers. 



No. 170.— Redness of skin of abdomen; nothing at places of inoculation; spleen 

 enlarged, friable, full of blood; abdomen, thorax, and pericardial cavity contain 

 much yellow serum, congestion of the lungs with darker hemorrhagic foci through- 

 out; anterior lobes collapsed; kidneys enlarged, with a few extravasations on surface 

 and in parenchyma; mucous membrane of stomach and intestines covered with 

 many hemorrhagic points and spots. In large intestine, including rectum, numer- 

 ous old ulcers, some 1 inch across. Lymphatics in general extensivelv congested. 



No. 178.— Died quite unexpectedly. At the place of first inoculation two ftrnj 

 whitish masses ; spleen enlarged , friable ; its substance contains hemorrhagic infarcts ; 

 extravasations beneath both serous surfaces of the heart; congestion of lungs, ^vith 

 numerous darker hemorrhagic foci; lymphatic glands of abdominal cavity very dark 

 and gorged %vith blood; extensive ulceration about the ileo-ceecal -^Ive, in the 

 capcum, and colon; in the lower portion of colon and in the rectum numerous small 

 extravasations. Hemorrhage into pelvis of both kidneys. 



Thepost mortem, determination of a severe type of hog-cholera 

 in these four cases was confirmed by finding in the spleen of each 



