G48 



RSPORT OF THE COMMISSIONER OF AGRICULTURE. 



animal, by means of cover-glass preparations, numerous specific bac- 

 teria of this disease. Cultures in liquid media made from every 

 spleen were found pure when examined microscopically as v/ell as on 

 gelatine plates. This experiment likewise proved the inefficiency of 

 small quantities of non-attenuated virus introduced beneath the skin 

 in preventing an invasion of the micro-organisms from the aliment- 

 ary canal. 



A third lot of four pigs (Nos. 117, 171, 172, and 174), between three 

 and five months old, were inoculated as before with .2*^*^ each from 

 the second beef -infusion peptone culture derived from the spleen of 

 No. 159. On March 1 they were inoculated with .2'=" from the second 

 culture derived from the spleen of No. 156. In No. 117 there was a 

 slight swelling after the first and one as large as a chestnut after 

 the second inoculation. In No. 171 a mass 1^ to 2 inches long and 

 three-fourths inch in diameter was found at site of the first inocula- 

 tion. There was but a small nodule at the place of the second inoc- 

 ulation. In No. 172 two lumps, like small marbles, formed after the 

 first inoculation; after the second only a small nodule formed. In 

 No. 174 the reaction after the second inoculation was manifested by 

 an irregular tumor about 2 inches long and one-third of an inch in 

 diameter, the reaction at the place of the first inoculation being less 

 marked. 



Of these four, two (Nos. 117 and 172) were fed with the viscera of 

 pigs dead from hog-cholera, together with two control animals (Nos. 

 192 and 193), on March 19. The rest (Nos. 171 and 174) were simply 

 placed in the large infected pen March 22, with those that had been 

 ted with infectious matter. Below the result is given in a tabulated 

 form. It shows that all the animals succumbed to the disease, those 

 simply exposed by contact with the sick as well as those fed. Of the 

 inoculated animals, those fed died in twenty-one and eighteen days 

 after feeding; those exposed, in twenty-two and twenty-five days re- 

 spectively. Those not inoculated died twelve and eleven days respect- 

 ively after feeding. Here, likewise, we notice the prolongation of 

 life in the inoculated pigs. 



♦Checks. 



The lesions found at the autopsies of these pigs are briefly as fol- 

 lows: 



No. 117. — Extensive reddening of the skin of abdomen; gi-eat enlargement of spleen, 

 which is gorged with blood, very soft; petechial discoloratioiis on surface of lungs 

 and on section; large intestine studded with broad deep ulcers as far as the rectum; 

 a few in ileum. 



No. 171. — Skin over ventral aspect of body deeply reddened; hemorrhagic spots 

 under peritoneal covering of diaphragm and large intestine and under capsule of 



