BUREAU OF ANIMAL INDUSTRY. 



(149 



kidneys; lungs congested, containing numerous dark hemorrhagic lobules; part of 

 anterior lobes collapsed. The spleen very large, dark colored; nodes slightly raised 

 above surface, shown on section to be .hemorrhagic mfarcts; lymphatic glands gener- 

 ally highly congested; petechial spots on surface and in cortex of kidneys; hemor- 

 rhagic foci throughout mucosa of stomach and intestines. About four large ulcers 

 ii\ coecum and colon. 



No. 172.— Reddening of skin of ventral aspect of body and of subcutaneous tissue 

 generally; firm, pale yellovp- cheesy masses, surrounded by a thin membrane, at place 

 of inoculation; engorgement of spleen and lymphatic glands; extravasations in pa- 

 renchyma of kidneys. In ca3cum and colon numerous deep ulcers, some coalesced. 

 Mucosa of stomach generally congested, and that of intestines thickly dotted with 

 petechiaa. 



No. 174. — Deep reddening of skin of abdomen; encysted cheesy mass at site of first 

 inoculation; great enlai-gemcnt of spleen; prominent red points on surface; effusion 

 into abdominal cavity; anterior lobes of lungs collapsed, remainder normal; lymphat- 

 ics highly congested; three large ulcers in c^cum; valve thickened and ulcerated; 

 petechise numerous throughout mucosa of stomach and intestines. 



No. 192. — Control animal. Reddening of skin of ventral aspect of body and of 

 subcutaneous tissue; spleen swollen, full of blood, friable. Atelectasis of tlie small 

 anterior lobe of each lung; ulcers on the mucous surface of gall bladder. Cortex of 

 lymphatic glands congested; mucosa of large intestines congested; numerous ulcers 

 in caecum and upper colon. 



No. 1 93. — Subcutaneous connective tissue considerably reddened ; spleen but slightly 

 enlarged, not much softened. Mucous membrane of stomach, of large and small in- 

 testines, deeply congested; contents of large intestine fluid, chocolate colored. 



In cover-glass preparations from the spleen pulp of these animals 

 numerous bacteria of hog-cholera were found in each preparation. 

 Both gelatine and liquid cultures from every spleen proved to be 

 pure cultures of the bacterium of hog-cholera. 



The diagnosis made on post mortem was thus confirmed by micro- 

 scopic examination and culture. 



To determine the effect of a single inoculation, on February 13 two 

 igs (Nos. 115 and 160) received subcutaneously each 1*'*' of the second 

 eef -infusion peptone culture obtained from the spleen of a pig. In 

 No. 115 a tumor as large as a marble was found at the seat of in- 

 oculation March 9. In No. IGO tlie tumor was elongated, about 3 

 inches long and three-eighths of an inch thick. No. 115 was fed with 

 viscera taken from cases of hog-cholera March 19. No. 160 was 

 simply exposed to the disease by being transferred to the large in- 

 fected pen. No. 115 died April 8. No. 160 recovered and was well 

 May 6. The detailed account of this experiment is appended : 



Post mortem iiotes.—No. 115.— Firm, pale yeUow tumor at seat of inoculation, 

 encysted; center imdergoing softening. Spleen tumefied, very dark and friable. A 

 few extravasations beneath serous coverings of heart. In cortex of kidneys numer- 

 ous hemorrhagic points; cystic degeneration of right kidneys; advanced ulceration 

 of caecum and colon; scattered petechiee in mucosa of stomach and small intestine. 



No. 160.— -Was very low for a time, beginning with April 1. It was barely able to 

 stand and its appetite was poor. It rapidly recovered, however, and was gaining 

 flesh in May. Whether tlie animal was suffering from hog-cholera or from the 

 Sclerostoma pinguicola (kidney worm), with which some of this lot were found 

 affected, cannot be said. 



In order to determine whether a single injection of a comparatively 

 large quantity of culture liquid, while not inducing the disease, would 



