652 



REPORT OF THE COMMTRSTONER OP AGRICULTURE. 



1*=*=, l|cc^ o'^^^, and 2^'='', of the culture liquid respectively. No un- 

 toAvard results following the injection of these large doses, they were 

 transferred to the large infected pen May 25. 



A second lot (ISTos. 226, 228, 215, and 229) w^ere treated in precisely 

 the same way and at the same time, excepting in receiving ^^° for 

 the first dose instead of i^°. 



* Checks. 



No. 214 being in a dying condition July 1, was killed. In the cEecum and colon 

 were found very large, deep, blackish ulcers upon a pale mucosa. The case was evi- 

 dently one of chronic hog-cholera. A pure Uquid culture of the hog-cholera bacte- 

 rium was obtained from the spleen. 



No. 227 died June 27. The lymphatic glands were deej)ly congested; the mucosa 

 of large intestine was generally pigmented and covered with large blackish ulcers. 

 Small yellowish ulcers Avere also found in the ileum. The points of injection were 

 occupied by encysted, partly liqueiied masses. 



No. 223 was found dead July 2. At the points of injection encysted masses were 

 found, the contents of one of which were discharging tlirough an opening in the 

 skin. The mucosa of the entire large intestine deeply congested. Scattex'ed ulcers 

 of varying age and size in the caecum and colon. Bacterium in spleen. 



No. 222, after a period of unthriftiness, was found dead July 1. The autopsy re- 

 vealed a clu'onic broncho-pneumonia, with pleuritic adhesions of right lung. The 

 mucous membrane of the caecum and colon, besides being studded with a large num- 

 ber of broad shallow ulcers, was deeply and uniformly congested, the congestion 

 involving also the lower portion of the ileum. On both thighs an encysted semi- 

 liquid mass indicated the seat of the inoculation. This case suggests the probability 

 of a double infection, the first represented by the ulceration, the second by the more 

 recent inflammation of the mucous membrane. 



Of the second lot, which had received i" of the first inoculation, all succumbed to 

 the infection. No. 226 died July 3. The characteristic lesion was extensive ulcera- 

 tion, together with deep congestion of the mucosa of large intestine. Encysted 

 masses at the points of inoculation. A considerable number of bacteria of hog- 

 cholera in the spleen. 



No. 228 died July 13. In this animal the mucosa of caecum and colon presented 

 a continuous mass of necrosed blackish tissue, the ileo-C£ecal valve being enlarged 

 to twice the nomal size. A few scattered yellowish ulcers in the lower portion of 

 the colon. No. 215 died July 10, probably affected in the same way, though no post 

 mortem examination was made. 



No. 229 died June 27. In this case the lymphatic glands were in general deeply 

 congested; ecchjTuoses beneath the serous membranes. Pigmentation of the mu- 

 cous membrane of the stomach, duodenum, ileum, and large intestine from former 

 extravasations. Several large ulcers on the valve and some otliers in colon. Ulcers 

 in the cardiac portion of the stomach. Encysted masses at the point of inoculation. 



Nos. 224 and 225 were penned with the above eight animals as checks. No. 225, 

 after being siclr for a few days, was found dead June 27. The mucosa of the caecum 

 • and upper half of the colon is extensively pigmented and ulcerated, the lower half 

 deeply congested. The ileum is also ulcerated for 5 or 6 feet from the valve. Many 

 of the ulcers are so deep as to have produced inflammation of the serous membrane 

 and thickening of the intestinal walls. The other check (No. 224) hved over two 

 months after exposure, being xmthrifty during this i)eriod. On j^ost mortem the 

 mucosa of large intestine was considerably pigmented and scars of healed ulcers 

 were present. A large suppurating wound of the lower jaw, involving the bone, 

 may have contributed towards the fatal issue. 



