BUREAU OP ANIMAL INDUSTRY. 663 



softening; the connective tissue and fat of the whole body of a deep 

 yellow color; liver very firni, bloodless, and of a peculiar yellowish 

 red color throughout; medulla of kidneys deeply reddened; two large 

 cysts in the right one. In neither case was the alimentary tract dis- 

 eased. In both there was cirrhosis of the liver, producing in the 

 second animal a general jaundice. From neither were cultures of 

 the inoculated microbe successful, though blood from the heart, the 

 spleen, and the liver were used. The tubes remained sterile. 



Nos. 288 and 390, which had been retained in the same pen, did not 

 contract the disease from the others, as would ordinarily happen in 

 hog-cholera. No. 288 was fed with hog-cholera viscera October 12, 

 and died from the effects December 4; caecum and colon ulcerated. 

 No. 290, fed at the same time, died October 28, the only visible cause 

 of death being retention of urine. 



This microbe was, therefore, fatal to mice, rabbits and pigs, pro- 

 ducing in the pig an acute inflammation of the liver, leading to a 

 marked cirrhosis and general jaundice. 



The same disease found near Sodorus, III. — The same microbe 

 was obtained from an outbreak in Sodorus, 111., several months 

 later. On page 630 a description is given of two post mortems, in 

 one of which (No. 1) the lesions were ulceration of the large intestine 

 and a grayish hepatization of the lungs. From this animal the bac- 

 terium of hog-cholera had been obtained from the spleen. In _ the 

 other animal (No. 2) the lung lesions only were ijresent. Portions 

 of the solidified lung tissue from No. 2, hardened in strong alcohol, 

 were submitted to a microscopic examination. The tissue was infil- 

 trated with parajBfine, the sections treated with turpentine to remove 

 the imbedding substance, and then stained in various ways. The 

 smaller bronchi and air-cells were completely filled with an exudate, 

 consisting of white blood corpuscles chiefly, and some larger pale 

 cells, probably derived from the ei)ithelium. This infiltration was 

 exceedingly dense in many places; in others less so. The septa or 

 alveolar walls were not perceptibly affected, but the capillaries were 

 distended with blood corpuscles, and formed an unstained mesh-work 

 around the deeply stained alveolar contents. The interlobular con- 

 nective tissue was also infiltrated, and the lymphatic spaces distended 

 and filled with a fibrillar network of coagulated lymph. When the 

 alveolar contents were carefully examined with a one-eighteenth 

 homogeneous objective, after staining the section in Loffier's alkaline 

 methylene blue for several hours and decolorizing in one-half per cent, 

 acetic acid, groups of very minute oval bacteria were recognizable, in 

 size and outline like those obtained in cultures from the pleura. These 

 groups were very large and extended through the depth of the section, 

 a fact easily recognized by focusing up and down. They were found 

 in all parts of the section, the bacteria themselves and the groups 

 they formed being readily recognizable. No other bacteria could be 

 detected, though the sections were searched over many times. Stain- 

 ing in aniline water methyl violet overnight did not bring these 

 groups out so clearly as the stain above given. These groups, more- 

 over, were present in those air-cells chiefly in which the exudate was 

 but moderately dense. 



The lesions of the lungs found in both pigs at Sodorus, 111., were 

 different from those occasionally found in pos^ mortem examinations 

 of hog-cholera at the Washington Experimental Station. In the 

 latter the acute cases, characterized by hemorrhagic lesions in various 

 organs, usually presented lungs which were dotted with dark red 



