BUREAU OP ANIMAL INDUSTRY. 665 



In the other rabbit the local effect was equally extensive, and there was a more 

 marked grayish discoloration of the tliigh and contiguous abdominal muscles. Peri- 

 tonitis less marked ; no coagiila present. The bacteria not so numerous in the organs, 

 but very abundant in the peritoneal cavity. Liquid cultures from the latter and 

 from blood were pure. Gelatine tubes, cultures from the liver and spleen, contained 

 many colonies. 



Rabbits, mice, and pigeons were tlius shown susceptible when in- 

 oculated with the quantities above mentioned. Two rabbits were at 

 the same time inoculated with large quantities of two other microbes 

 obtained from spleens. Both remained unaffected. In one a small 

 circumscribed abscess could be seen through the skin. 



Of the two pigs inoculated (Nos. 330 and 331), No. 330, which had 

 received the larger dose (5^") died in nine days, after exhibiting the 

 same symptoms as those manifested by the two former cases — debil- 

 ity, loss of appetite, inflamed eyes. In this animal there was a sim- 

 ilar condition of the liver, together with a deep yellow staining of 

 the connective and adipose tissue generally. Cultures negative. 

 No. 331 died thirty-five days after inoculation. In this animal there 

 was a less pronounced pathological change in the liver. Icterus pres- 

 ent. No cultures were made. 



in order to confirm and extend the preceding ino(?ulation experiments a second 

 series was ijlanned in the same way. A beef infusion peptone culture, which had 

 been derived from a single colony of the microbe on a gelatine plate and was twenty- 

 four hours old, was used to inoculate of mice, pigeons, fowls, and white rats 3 each, 

 1 guinea-pig, and rabbit. The mice, which received -^'"^ beneath the skin of the 

 back, died in two and six days respectively. In the first one the spleen and blood 

 were crowded with bacteria, and a liquid culture from the blood proved pure. From 

 the second mouse no cultures were made; bacteria few or absent from the organs. 

 Of the 3 pigeoVis, 1 inoculated with l"" was dead next day. At the place of inoculation 

 in the pectoral the muscular tissue was whitish, parboiled for a depth of one-fourth to 

 one-half inch ; cultures from blood and liver sterile. The other pigeon, which received 

 ■i'=<=, survived. The white rats, receiving respectively i" and i'^" subcutaneously in the 

 thigh, did not prove susceptible. The rabbit, which received i"" in the same place, 

 died in three days, after showing symptoms like those in the preceding exijeriment. 

 Locally the lesions ^vere the same; thickening of the fascia more pronounced; lar- 

 daceous appearance of the surface of the muscular tissue; punctiform extravasa- 

 tions both on abdomen and on thigh as far as symphysis pubis. In the abdominal 

 cavity the serous surface of the entire intestine appeared as if sprayed with blood, 

 the extravasation being beneath the serosa and not visible from the mucous surface. 

 Small intestine but faintly reddened ; only a few delicate fibrils of exudation as yet 

 visible; a fev,- extravasations on capsule of kidney, which is deeply reddened through- 

 out; spleen and lungs normal; liver invaded by coccidmm ovifornie. Cover-glass 

 preparations of spleen, liver, and peritoneum contain the microbes in abundance; 

 in tlie peritoneal exudate they seem as numerous as in a liquid culture. Cultures 

 both in beef infusion and in gelatine from the blood and peritoneal fluid were pure. 



The guinea-pig, whicli had received into the thigh a rather large dose, i", suc- 

 cumbed on tlie sixth day. At the point of inoculation there is but a slight infiltra- 

 tion and thickening of the subcutaneous connective tissue. In the abdomen, both 

 spleen and liver somewhat enlarged. Covering these a thin, translucent, gelatinous 

 layer, easily scraped away, and particularly well marked on the liver. Lungs deeply 

 congested, not collapsed, but showing the impression of the ribs. The pleura cov- 

 ered with a similar exudate. The internal organs contained scarcely any bacteria, 

 but coyer-glasses touched to the surface of lungs and liver showed the exudate, 

 consisting chiefly of leucocytes, to contain large numbers of the injected bacteria. 

 Gelatine cultures of blood and from the liver, a liquid culture from the blood, were 

 pure. A liquid culture from the peritoneal surface of spleen contained also a mo- 

 tile bacillus. The contact of this organ with the very tliin-waUed large intestine 

 may explain the contamination. 



Of two fowls inoculated beneath the skin of the pectoral with |" and 1", respect- 

 ively, the one which received the smaller dose died in five days. In this bu-d the 

 local lesion was very extensive. On removing the thickened, discolored skin the 

 large pectoral was parboiled in appearance throiighout half its mass; the remainder 

 of the muscle studded with small extravasations. The pathological changes in- 

 volved also the smaller pectoral in points and patches, extending through one of 



