BUREAU OF ANIMAL INDUSTRY. 667 



the same microbe obtained from tlie pleura, the spleen, and the blood 

 of pig No. 2 of the same herd. In the other tube the bacterium of 

 hog-cholera was found. It will be remembered that pig No. 1 had 

 in addition to the ulcerations of the caecum and colon, partial hepa- 

 tization of both lungs, while No. 2 had merely the lung lesions. 

 About a month later a liquid culture, made from a gelatine-tube cult- 

 ure of this blood, was used to inoculate a rabbit, only gV being 

 injected subcutaneously into the thigh. The animal was found dead 

 on the third day. The lesions were nearly identical with those ob- 

 served previously after inoculation with this microbe— local thicken- 

 ing of the fascia, hemorrhagic markings of the muscular tissue, with 

 superficial degeneration at the point of inoculation, ecchymoses on 

 the contiguous abdominal walls, peritonitis indicated by a few 

 stringy deposits on liver, as well as more than the normal quantity 

 of serum. Cover-glass prei)arations of the spleen and liver revealed 

 a large number of the injected cocci, which, stained in alkaline 

 methylene blue, showed the polar stain very clearly. A cover-glass 

 touched to the serous surface of the large intestine contained immense 

 numbers of the same germ. Gelatine tube cultures of liver and 

 heart's blood grew in the characteristic manner. From the blood 

 culture a second rabbit was inoculated, as follows: The hair was 

 clipped from the inner aspect of the thigh, which was washed with 

 . 1 per cent, solution of mercuric chloride. With a flamed lancet a 

 little pocket was formed beneath the skin, into v;hich a small quan- 

 tity from the gelatine culture was introduced on a platinum loop. 

 The animal sat quietly in the coop, eating but little, and breathing 

 slowly for some days before death, which occurred ten days after 

 inoculation. 



Slight enlargement of the inoculated thigh. Subcutaneous tissue over the inner 

 and caudal aspects of the thigh, on the abdomen beyond umbihcus, greatly thick- 

 ened by inflammatory infiltration, which is of a soft, pasty consistency, grayish 

 white. It is closely adherent to skm, but not to muscular tissue on abdomen, from 

 which it may be easily removed. The muscular tissue beneath is dotted with punc- 

 tiform extravasations. On the caudal aspect of the thigh the infiltration is closely 

 adherent to muscular tissue as far as the pubis, involving the superficial muscular 

 fibers, which are whitish, softened; the groin stained with a frothy, blood-stained 

 serum; the superficial lymphatic gland on the same side enlarged to'size of a horse- 

 chestnut; on section wliitish, lardaceous; the lobules of the gland appearing as pale 

 red masses imbedded in it. The serosa of intestines and the liver coated with a 

 glairy, translucent, grayish deposit, which maybe drawn out into threads and peeled 

 from the liver in a thin layer. This exudate" contains very few cells, but immense 

 numbers of bacteria, evidently in a state of active multiplication, as thev are quite 

 small, resembling oval cocci, and staining uniformly. Parenchyma of liver uni- 

 formly dark l)ro\vnish: bile very dark gi-eenish; spleen not enlarged: lungs normal; 

 right heart filled witli a translucent gelatinous clot; left, with dark liquid blood; 

 mucosa of stomach, which contains considerable food, coated with a tenacious mu- 

 cus; cu-cumscribed hemorrhagic spots scattered over the right half of the stomach; 

 very few bacteria in the parenchyma of spleen and fiver. 



In thi.s case ten days elapsed between inoculation and death. The 

 local lesion was very extensive, the peritonitis advanced. According 

 to previous experience the difference between this and the preceding 

 case was due entirely to the mode of inoculation. There is also an- 

 other difeerence worthy of note. In the preceding rabbit the bacte- 

 ria showed very beautifully the stain at the ends of the short rod; in 

 this one they did not show it clearly at all. 



This microbe was therefore present in the pleural cavity, in blood 

 from the heart, and in the spleen of one pig, and in the blood of an- 

 other. It IS highly probable, judging from the microscopic exam- 



