BUREAU OF ANIMAL INDUSTRY. 669 



tion contained the microbe in abundance, but cultures from the blood 

 and liver remained sterile. It seems that it has not the power to mul- 

 tiply in the internal organs, though producing exceedingly severe 

 local lesions sufficient to cause death. The lesions which the sub- 

 cutaneous injection of the microbe produces in pigs were so uniform 

 and yet so peculiar, that it seemed necessary to add a few more experi- 

 ments to those already made. 



November 18, two pigs (Nos. 374 and 375) received hypodermically 

 5'^'' (one-half into each thigh) of a beef -infusion peptone culture two 

 days old, derived from a gelatine culture (rabbit) about one month 

 old. Three pigs were placed with these to determine whether the 

 disease was infectious. In both animals two days after inoculation 

 the sclerotic became deeply reddened. This congestion was followed 

 by discharge, which gummed the lids together for a part of the time. 

 In about a week these symptoms gave way and the eyes became jaun- 

 diced. The eyes of the three check pigs were not affected. Xo. 375 

 died November 25, one week after inoculation. The»subcutaneous 

 connective tissue of a deep yellow color. The points of inoculation 

 occupied by cysts filled with a blood-stained serum. Blood black, 

 partially coagulated. Hypostatic congestion of lungs. Purplish 

 spots beneath pleura and in parenchyma; some lung worms present. 

 Liver very pale, bloodless, very tough. The sclerosis general and 

 the contraction of the connective tissue has made the caudal aspect 

 very concave. Removed from the body it resembles india-rubber, as 

 it retains the same form in whatever position it is laid. Gritty sen- 

 sation when cut. The gall bladder filled with semi-liquid, dark brown 

 bile, resembling plum juice, surrounding a mass of putty-like con- 

 sistency and of the same color. The papillar opening of the common 

 duct into the duodenum contained a plug of gelatinous mucus, and 

 when the duct was slit open it contained mucus only. The walls were 

 not even bile-stained; the secretion of bile had ceased some time past. 

 When the liver was cut the section was of a dirty reddish-yellow 

 color throughout; no blood flowed from the vessels; when scraped the 

 cellular elements of the acini came away readily, leaving the tough 

 interlobular tissue in situ as a honey-combed mass. 



Sections hardened in alcohol and stained in alum-carmine showed 

 a large aniount of connective tissue as compared -with the normal 

 liver. This increase was general. In the parenchyma of the lobules 

 there were circumscribed areas in which the protoplasm of the cells 

 stained very feebly, while the nuclei were either shriveled or else re- 

 placed by a group of granules. The characteristic trabecular struct- 

 ure in these areas was more or less destroyed. Almost everv lobule 

 examined contained these altered regions, which were situated as a 

 rule near the periphery. 



There was a very marked venous stasis of the portal circulation, 

 characterized by an overdistension of the vessels, bringing even the 

 smallest into view. The vasa recta of kidneys very prominent, giving 

 the pyramids a bright red appearance. Serum in the abdominal 

 cavity of a deep yellow. This yellow tinge is present in the fat around 

 the base of the heart. The urine deep yellow, the mucosa of bladder 

 stained with the same color. The urine readily gives, with Gmelin's 

 test, the colors characteristic of the bile pigments. The intestinal 

 tract normal throughout, save what changes arose from the general 

 stasis of the portal circulation. The stomach empty and coated with 

 a ^dscid mucus. 



The injected microbe which without doubt caused these lesions 



