Pinkie 
74 ROCHESTER ACADEMY OF SCIENCE, [June 23,. 
Saucer. 20 non-liquefying colonies. 
4 liquefying colonies. 
Plate 1. 12 colonies. 
Plate 2. 10 colonies. 
Plate 3. 12 colonies. 
The colonies were chiefly colored or raised white and porcelain-like in appearance... 
An average of twelve colonies to each five drops. 
The pipette used measured 25 drops to I c. c. and therefore the water contained» 
about 60 colonies to each c. c. 
There were five gelatine needle cultures made and an examination of them showed 
that two were sterile, and three contained micrococci. : 
A summary of the work upon these first samples of water shows that : 
Nos. 1 and 2 contained very numerous colonies, many of them liquefying-. 
No. 3 contained 800 colonies to the c. c. with numerous liquefying. 
No. 6 contained very numerous colonies with many liquefying. @ 
Nos. 8 and 9 the same. 
No. 11, 1000 colonies to the c. c. with many liquefying. 
No. 15, 4000 colonies to the c. c. with many liquefying. 
No. 16, 60 colonies to the c. c., chiefly color forming with a few liquefying- 
November 13, 188g, a second batch of waters was received from Rochester and submitteé 
at once to the same sort of analysis as the preceding. From each specimen a. 
gelatine saucer-culture was made—ten drops, and five drops to the culture. 
Three gelatine plate cultures were also made from each specimen—two drops to- 
each culture. 
All the cultures were made on Nov. 13. 
WaTER AA.—(Whitlock well.) 
Noy. 15—Saucers I and 2 not examined because there were too many~ 
colonies. 
Plate 1. About 50 colonies in each square of the counting plate, 
—59 squares in the plate—giving about 3000 colonies- 
in each two drops. The colonies were chiefly small. 
fine round, brownish in color with a few white and 
opaque, and eleven liquefying—no odor. 
Plates 2 and 3 were very similar to plate 1. 
The pipette used measured 24 drops to the c. c., and the water therefore contained 
36,000 colonies to the ec. c. 
There were 17 gelatine needle-cultures made. 
November 20, examination of these cultures showed that two were sterile, two- 
were liquefying (no odor) and that thirteen were made up of micrococci. 
WATER BB.—(Densmore well.) 
Nov. 15—Plate 1. Vo liquefying colonies. 
17 large white colonies. 
520 non liquefying colonies. Most of the colonies~ 
appear the same—small, round, white. 
Piate 2. Wo liquefying colonies. 
24 large white colonies. 
622 non-liquefying colonies. 
