116 Proceedings of Indiana Academy of Science. 
A. An ice box, temperature around 45° F. 
B. In a cold storage room in a creamery, temperature 36° F. to 
2 ie 
C. Cold room in creamery, temperature between 27° F. and 30° F. 
The counts obtained from these tests are given in Table I. 
TABLE I. 
Changes in Bacterial Content of Soil Stored in Different Refrigerating Rooms. 
Lengths of Time of Incubation Temp. 45° F Temp. 39° F Temp. 29° F 
0 days 12.4* 12.4 12.4 
21 Where 9.5 5.5 
78 0.7 4.8 4.5 
*Figures are millions per gram of soil as used. 
SUMMARY. 
It is known to be difficult to get accurate figures of the numbers of 
bacteria present in frozen soil. It is not known that the layer of soil 
just below the constantly increasing layer of frozen soil is not very 
favorable for the multiplication of certain classes of bacteria. 
The data reported in this paper, obtained in this laboratory and from 
the work of others does not prove that the number of bacteria present 
in soil is increased when the soil is frozen. 
