228 
The activity of these cultures is prolonged by covering the medium with 
sterile paraffin oil and chilling (0-10° Centigrade) just before their max- 
imum growth (five to seven days) is reached. 
These cultures are pathogenic, altho, apparently their virulence is lowered 
as indicated by a longer period of incubation and no fatalities. 
The cultural type differs from the normal blood type of the spirochaete 
morphologically and in its staining properties, 
This culture of Spirochaeta Novyi has been perpetuated upon ascitic 
fluid containing undefibrinated normal blood for six generations. 
REFERENCES. 
1. Deutsch. Med. Wehnschi., 1909 XXXYV, 835. 
ibid., 1260. 
ibid., 1261. 
Klin. Jahr., 1910, XXiii, 339. 
8. Compt. rend. Soe. de biol., 1910 LXViii, 684. 
4. Ztschi. f. Hyg. u. Infectionschrankh., 1911 LXViii, 27. 
5. Deutsch. Med. Wehnschi., 1911 XXXVii, 682. 
6. Jour. of Exper. Med. 1911 XIV, 99. 
7. ibid., 1912, XV, 199. 
8. ibid., VG Oke 
9. ibid., XV, 466. 
10. ibid. XVI, 194. 
a ides XVI, 261. 
12. bles XVI, 620. 
3. Proc., Ind. Acad. of Sci. 1919. 223 
14. ibid. 1916 267 
15. ibid., 266 
