BUREAU OF ANIMAL INDUSTRY. 481 



cyos. It was found dcnd the next mornin^f, or about four days after in- 

 oculation. Portions of the spleen, lirer, and blood from the heart were 

 rubbed on cover-glasses, dried and stained for half a minute in an aque- 

 ous solution of methyl-violet. Two cultures, one in gelatine and one in 

 In-ef broth peptone, had been previously made. On the three cover- 

 .mbisses very hue bacilli were found quite abundantly, some free and 

 some within white blood corpuscles (Plate II, Fig. 5). In one cell, at 

 h-ast thirty couhl be counted. Tiie preseuct of large numbers of bacilli 

 ill white blood corpuscles from the spleen of mice in which septicogmia 

 hiid been produced by the injection of putrid blood was })ointed out as 

 f;ir back as 1S7S by Koch, and lately confirmed by Lolfler and Schiitz, 

 iu mice inoculated with the virus of rouget. The culture in gelatine ot 

 blood from the heart assumed i)reciscly the same appearance as did the 

 culture from the gelatiue plates. The liquid culture proved to be made 

 up entirely of the fine bacilli, while its microscopic appearance coin- 

 cided with the liquid cultures from the gelatine i)lates. October 24, an- 

 other mouse showed symptoms of illness. The eyes were slightly suf- 

 fused, the coat staring, and the respiration labored. It lived through 

 the next day, but was found dead on the morning of the 2Cth, nine days 

 after inoculation. In tl:e liver and blood a moderate number of bacilli 

 were found ; in cover glasses of the spleen they were not observed. A 

 culture of blood from the heart in beef-broth peptone contained, two days 

 later, a pure culture of the slender bacilli. The third mouse remained 

 welL Inoculations with the pure cultures of the vaccine were not at- 

 tempted for want of time. Tlie above illustrates very clearly how in 

 certain cases pure cultures may bo obtained from impure mixtures by 

 simply inoculating with the mixture an animal susceptible to one of the 

 forms only. 



The foregoing experiments seemed to us sufficient proof that the mice 

 had died from a multiplication in the various organs of tl^e bacillus 

 found in Pasteur's vaccine. We were also convinced that the bacillus 

 cultivated as a vaccine in France was identical with the bacillus re- 

 garded as tlie cause of rouget in Germany. 



(h) Yaccination of pigs. — October IG, p'igs 78 and 94 were inoculated, 

 each with five drops of the first vaccine, one-half into each thigh j oSTos. 

 70 and 05 received in the same manner about 2'='^ each of the same vac- 

 cine. Pig 78 died October 20. Having a black skin discolorations 

 could not be made out. In tlie peritoneal cavity a very large quantity 

 of Coagulable, straw-colored lymph was found ; a fibrinous exudate cov- 

 ered the coils of the large intestine and lunips of the same lay loose in 

 the cavity; the peritoneum itself was pale. The live-r was of a pale 

 flesh color and almost bloodless, the lobules standing out very distinct. 

 A flbriuous exudate matted the different lobes together. Lungs of a 

 rosy colo]', slightly congested, some serum in the pleural sacs as well as 

 in the pericardial cavity. Bight heart distended by a clot, left empty. 

 In the stomach an intensely red patch was found near the pylorus cov- 

 ered with a whitish mucous layer, readily removed. The intestines ap- 

 parently healthy. The inguinal glands of one side were considerably 

 reddened. In order to see how far the inoculation with a few drops of 

 the vaccine was accountable for death, cover-glasses upon which bits of 

 the spleen and liver had been rubbed and others upon which blood and 

 serum from the peritoneal cavity had been dried were examined, but 

 they contained no bacteria of any kind. Cultures in gelatine tubes 

 were prepared with a platinum loop from the peritoneal, pericardial, 

 and pleural effusions from the cut surface of spleen and blood from the 

 heart. A portion of the spleen was dropped into a liquid culture at 

 31 AG— '85. 



