488 REPORT OF THE COMMISSIONER OF AGRICULTURE. 



rinm and another microbe growing in colonies, "wliich differed from the 

 former only in a want of color and opacity. Thus two of the three cult- 

 ures v\-ere impure, as anticipated, but all contained the same motile 

 microbe. 



In order to test the matter more thoroughly a number of mice were 

 inoculated November G, as follows : After chloroforming, a portion of tlio 

 .skin near the root of the tail on the back was freed from hair, the skin 

 cut with scissors, and a small portion of tissue introduced into the 

 j)0cket thus formed. Three mice received in this way a small ulcer 

 from the large intestine, two each a bit of hepatized hmg tissue, spleen. 

 Mild kidney. Those inoculated with the same kind of tissue were kept 

 in the same jar. 



One of the mice (Xo. 3) inoculated Avith a bit of kidney became ill 

 i!^ovember 9 (on the fourth day); it had a staring coat, eyes open, not 

 suffused ; dead next morning. There was no reaction at the site of 

 inoculation, no serous infiltration of tlio subcutaneous tissue; lym- 

 phatics of the slvinfold of tlie groin dark red, also spleen : liver very 

 pale, almost bloodless ; bladder greatly distended with urine. Cover- 

 glasses of kidney, spleen, liver, lungs, and blood from heart contain 

 fine bacilli, resembling verj' closely in disposition and size those which 

 were found in mice inoculated with Pasteui''s vaccine. 



A liquid culture, prepared from the blood, w;is turbid on the follow- 

 ing day. It was found to contain a large bacillus, besides the delicate 

 bacillus, which appeared in long filaments. As the former rapidly lique- 

 fied gelatine on plates, and an isolation was found impossible without 

 much estra labor, three mice were inoculated Xovi'mber 12 with about 

 five drops each of this culture in order to obtain a pure culture in this 

 way. One mouse (Xo. 8) died on the following day. All internal or- 

 gans were crowded with large bacilli. This fact, taken together with 

 the ccdamatous condition of the subcutaneous tissue of abdomen, led 

 us to infer malignant oedema as the cause of death. On iN^ovember 14 

 a second mouse (No. 11) was found dead. There was a sero-sanguin- 

 eous effusion, as in the iirst case, and in all the organs hirge bacilli 

 were found, but in addition, the fine bacilli were present in enormous 

 numbers, especially within tlie cells. In the blood long, wavy filaments 

 of the large bacillus were found, often interlaced. The third mouse 

 (No. 12), being very sick, was killed with chloroform. No cedema, no 

 large bacilli, but the fine bacilli in immense numbers. From the blood 

 of the heart two pure cultures in beef broth peptone were obtained, in 

 which the bacilli appeared November IG as wavy, curled, or angular 

 filaments. The contaminating bacillus produced in the gelatine cult- 

 ures a surface liquefaction and cloudiness of the gelatine, whife the 

 colonics of the same baciUus below the surface in the track of the 

 needle appeared as minute, roundish specks, not liquefying the gelatine. 



The culture in nutritive gelatine developed in a characteristic manner. 

 From the track of the needle faint cloud-like processes extended later- 

 ally almost to the sides of the tube, so as to impart to the entire gela- 

 tine a cloudy appearance. The cultures of the bacillus of rouget were 

 different in that the growth remained near the needle track, and hence 

 was denser. This difference is also mentioned by foreign investigators. 

 {Lbjjici\ ArJ)eitcn a. d. Kaiserliclien Gcsiaulheitsamte, Bd. I, S. 47.) 



We give these results in detail, because it is the only time in our work 

 in which the fine bacilli resembling rouget made their appearance, either 

 in cultures or in the experimental animals ; and their growth in gela- 

 tine sufficiently disproves their identity with the bacilli of rouget. 



Of those inoculated with the ulcer, one died ou the tenth day, the 



